ADAM17/TACE 蛋白, Rat (HEK293, His)

ADAM17/TACE Protein, a pivotal enzyme, cleaves TNF-alpha and activates various cell-surface proteins like p75 TNF-receptor. It also mediates the cleavage of Notch, activates the Notch pathway, and contributes to hemostasis by shedding GP1BA. ADAM17/TACE further facilitates the cleavage of LAG3, IL6R, and FCGR3A, showcasing its significance in diverse cellular functions. ADAM17/TACE Protein, Rat (HEK293, His) is the recombinant rat-derived ADAM17/TACE protein, expressed by HEK293 , with C-10*His labeled tag. The total length of ADAM17/TACE Protein, Rat (HEK293, His) is 546 a.a., with molecular weight of 57 & 20 kDa, respectively.

ADAM17，也称为 TACE，在多种细胞过程中充当关键酶，特别是将 TNF-α 的膜结合前体裂解为其成熟的可溶形式。除了在 TNF-α 加工中的作用外，ADAM17 还表现出广泛的底物特异性，协调各种细胞表面蛋白的蛋白水解释放，例如 p75 TNF 受体、II 型白介素 1 受体、p55 TNF 受体、转化生长因子-α、 L-选择素、生长激素受体、MUC1 和淀粉样前体蛋白。此外，ADAM17 通过介导 Notch 裂解，生成称为 Notch 细胞外截短 (NEXT) 的膜相关中间片段，充当 Notch 途径的激活剂。它通过脱落血小板糖蛋白 Ib α 链 (GP1BA) 并介导 LAG3、IL6R 和 FCGR3A 的裂解而参与止血，凸显了其在多种细胞功能中的重要性。

Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 and the specific activity is 250-2200 pmol/min/µg.

Rat

HEK293

C-10*His

Q9Z1K9 (P18-D563)

57027  [NCBI]

PRPPEEPGSGSHLRLEKLDSLLSDYDILSLSNIQQHSIRKRDLQSATHLETLLTFSALKRHFKLYLTSSTERFSQNLRVVVVDGKEESEYSVKWQDFFSGHVVGEPDSRVLAHIGDDDVTVRINTDGAEYNIEPLWRFVNDTKDKRMLVYKSEDIKDFSRLQSPKVCGYLNADSEELLPKGLIDREPSEEFVRRVKRRAEPNPLKNTCKLLVVADHRFYKYMGRGEESTTTNYLIELIDRVDDIYRNTSWDNAGFKGYGVQIEQIRILKSPQEVKPGERHFNMAKSFPNEEKDAWDVKMLLEQFSLDIAEEASKVCLAHLFTYQDFDMGTLGLAYVGSPRANSHGGVCPKAYYNPGVKKNIYLNSGLTSTKNYGKTILTKEADLVTTHELGHNFGAEHDPDGLAECAPNEDQGGKYVMYPIAVSGDHENNKMFSNCSKQSIYKTIESKAQECFQERSNKVCGNSRVDEGEECDPGIMYLNNDTCCNSDCTLKPGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGDAED

57&20 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.