AG-2 蛋白, Human (HEK293, C-His)

AG-2 protein is critical for MUC2 post-transcriptional synthesis and secretion, implicating its role in intestinal mucus production. As a proto-oncogene, AG-2 affects cell migration, differentiation, and growth. AG-2 Protein, Human (HEK293, C-His) is the recombinant human-derived AG-2 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of AG-2 Protein, Human (HEK293, C-His) is 155 a.a., with molecular weight of ~20 kDa.

AG-2 蛋白对于 MUC2 的转录后合成和分泌至关重要，表明其可能参与肠道细胞的粘液产生。除了在粘蛋白调节中的作用外，AG-2 还作为一种原癌基因出现，对细胞迁移、分化和生长产生影响。值得注意的是，AG-2 促进细胞粘附，强调其对细胞过程的多方面贡献。在结构上，AG-2以单体和同二聚体的形式存在。此外，它还与 LYPD3 和 DAG1 (alphaDAG1) 相互作用，形成可进一步调节其功能的复合物。与 MUC2 的相互作用以二硫键为特征，凸显了 AG-2 在细胞环境中建立的复杂分子关系。

Measured by the ability of the immobilized protein to support the adhesion of PC-3 human prostate cancer cells. The ED₅₀ for this effect is 1.963 μg/mL, corresponding to a specific activity is 509.424 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of PC-3 human prostate cancer cells.  The ED₅₀ for this effect is 1.963 μg/mL， corresponding to a specific activity is 509.424 units/mg.

Human

HEK293

C-10*His

O95994 (R21-L175)

10551  [NCBI]

RDTTVKPGAKKDTKDSRPKLPQTLSRGWGDQLIWTQTYEEALYKSKTSNKPLMIIHHLDECPHSQALKKVFAENKEIQKLAEQFVLLNLVYETTDKHLSPDGQYVPRIMFVDPSLTVRADITGRYSNRLYAYEPADTALLLDNMKKALKLLKTEL

Approximately 20 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.