1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. aKMT 蛋白, Sulfolobus islandicus

aKMT 蛋白, Sulfolobus islandicus

目录号: HY-P701940
产品使用指南

赖氨酸甲基转移酶 (aKMT) 负责催化靶蛋白中赖氨酸残基的甲基化,使用 S-腺苷-L-甲硫氨酸 (SAM) 作为甲基供体。该酶表现出广泛的底物特异性,在体外表现出主要在穹窿染色质蛋白 Cren7 的“Lys-11”、“Lys-16”和“Lys-31”处以及几种重组硫化叶菌蛋白的甲基化能力。aKMT 蛋白, Sulfolobus islandicus 是重组的 aKMT 蛋白,由 E. coli 表达,不带标签。aKMT 蛋白, Sulfolobus islandicus 全长 160 个氨基酸。

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描述

Lysine methyltransferase (aKMT) is responsible for catalyzing the methylation of lysine residues in target proteins, using S-adenosyl-L-methionine (SAM) as a methyl donor. The enzyme exhibits broad substrate specificity, showing methylation primarily at "Lys-11", "Lys-16" and "Lys-31" of the vault chromatin protein Cren7 as well as several recombinant Sulfolobus proteins in vitro Ability. aKMT Protein, Sulfolobus islandicus is the recombinant aKMT protein, expressed by E. coli , with tag free. The total length of aKMT Protein, Sulfolobus islandicus is 160 a.a., .

研究背景

aKMT 蛋白充当赖氨酸甲基转移酶,催化靶蛋白中赖氨酸残基的甲基化。这种酶活性是使用 S-腺苷-L-甲硫氨酸 (SAM) 作为甲基供体来完成的。aKMT 显示出广泛的底物特异性,并且能够甲基化各种靶蛋白。特别是,已观察到在特定的赖氨酸残基(包括“Lys-11”、“Lys-16”和“Lys-31”)处甲基化了穹窿染色质蛋白 Cren7。此外,aKMT 表现出序列无关的赖氨酸甲基化,展示了其在体外修饰一系列靶蛋白方面的多功能性。

种属

Others

表达系统

E. coli

标签

Tag Free

蛋白编号

F0NBH8 (S2-K161)

基因 ID

8761611

蛋白结构
N-term
aKMT (S2-K161)
Accession # F0NBH8
C-term
同用名
Protein-lysine N-methyltransferase; Archaeal protein lysine methyltransferase; aKMT
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

aKMT Protein, Sulfolobus islandicus 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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