ALK-7 蛋白, Rhesus Macaque (HEK293, Fc)

ALK-7 is a type I receptor serine-threonine kinase mediate inhibitory as well as stimulatory signals for growth and differentiation by binding to members of the TGF-β superfamily. ALK-7 combined with specific ligands, such as Nodal, activin B and growth differentiation factor (GDF), can activate Smads and other signaling pathways, thereby regulating cell proliferation, differentiation and apoptosis in various cells^[1][2][3]. ALK-7 Protein, Rhesus Macaque (HEK293, Fc) is produced in HEK293 cells with a C-Terminal Fc-tag. It consists of 89 amino acids (G25-E113).

ALK-7，也称为ACVR1C，是一种与I型受体特征一致的丝氨酸/苏氨酸激酶。ALK-7 主要在中枢神经系统中表达。ALK-7 可以以配体依赖性方式与 TGF-β 和激活素的 II 型受体丝氨酸-苏氨酸激酶形成复合物^[1]。
ALK-7 基因编码 55- kDa 细胞表面蛋白，其激酶结构域与之前分离的 TGF-β 和激活素 I 型受体具有高达 78% 的氨基酸序列同一性。然而，在胞外域中，ALK-7 的差异更大，与 ALK 亚家族的所有成员表现出相当的相似性。ALK-7 mRNA 最初是从大鼠大脑中鉴定和克隆的，它存在于大鼠的整个消化系统和中枢神经系统中。ALK-7 作为 I 型受体的功能已通过激活 TGF-β/激活素反应报告基因的组成型活性突变体形式得到证实。还发现 ALK-7 可以激活胎儿和成年大鼠胰腺中 Smad 通路的某些成分，例如 Smad2 和 Smad3。在大鼠嗜铬细胞瘤PC12细胞系中，ALK-7不仅激活Smad2、Smad3以及细胞外信号调节激酶和JNK的MAPK，而且还抑制细胞增殖。人类 ALK-7 基因已被定位到 2q24.1-q3 的遗传位置，大部分 mRNA 位于大脑、胰腺和结肠中。ALK-7 通过激活 Smad2/3^[1][2][3] 介导高环境葡萄糖诱导的成肌细胞凋亡。
ALK-7与Nodal、激活素B、生长分化因子(GDF)等特异性配体结合，可激活Smads等信号通路，从而调节多种细胞的增殖、分化和凋亡。除此之外，ALK-7 与 ALK-5 和 ALK-6 一起参与肾间质纤维化^[1][2][3].

Activin AB binds ActRIIA-Fc with a high affinity, whereas activin AB shows little binding to ALK7-Fc or ALK4-Fc (.5-5 μg). An estimated Kd of activin AB to ActRIIA-Fc is 333 pM. The affinity of activin AB for ActRIIA-Fc/ALK7-Fc is greater than that for ActRIIA-Fc and has an estimated Kd of 125 pM. This indicates the enhanced activin AB binding to the activin receptors in the presence of ALK7^[5].

Rhesus Macaque

HEK293

C-hFc

F7GDQ6 (G25-E113)

697826  [NCBI]

Approximately 36.6 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.

• [1]. M Rydén, et al. A novel type I receptor serine-threonine kinase predominantly expressed in the adult central nervous system. J Biol Chem. 1996 Nov 29;271(48):30603-9.  [Content Brief]

  [2]. Byung-Chul Kim, et al. Activin receptor-like kinase-7 induces apoptosis through activation of MAPKs in a Smad3-dependent mechanism in hepatoma cells. J Biol Chem. 2004 Jul 2;279(27):28458-65.  [Content Brief]

  [3]. Wen-bo Li, et al. Silencing of activin receptor-like kinase 7 alleviates aortic stiffness in type 2 diabetic rats. Acta Diabetol. 2015 Aug;52(4):717-26.  [Content Brief]

  [4]. Kunihiro Tsuchida, et al. Activin isoforms signal through type I receptor serine/threonine kinase ALK7. Mol Cell Endocrinol. 2004 May 31;220(1-2):59-65.  [Content Brief]