Alpha-hemolysin 蛋白, S. aureus (P.pastoris, His)

Alpha-hemolysin, S. aureus, is a pore-forming toxin secreted by Staphylococcus aureus (S. aureus) and belongs to the cholesterol-dependent cytolysin (CDC) family. Alpha-hemolysin can disrupt corneal epithelial cell barrier function, inhibit epithelial cell migration and motility, and promote infection by enhancing the bacterial invasion of host cells. Alpha-hemolysin Protein, S. aureus (P.pastoris, His) is a recombinant Alpha-hemolysin protein expressed by P. pastoris yeast with N-6*His tag.

Alpha-hemolysin, S. aureus 是金黄色葡萄球菌 (S. aureus) 分泌的孔形成毒素，属于胆固醇依赖性溶细胞素 (CDC) 家族。Alpha-hemolysin 可在靶细胞膜上聚集成七聚体跨膜孔道，导致细胞裂解。Alpha-hemolysin 主要通过破坏角膜上皮细胞屏障功能，抑制上皮细胞迁移和活力，并通过增强细菌对宿主细胞的侵袭能力而促进感染。Alpha-hemolysin 的穿孔机制与膜穿孔引发的离子失衡和信号通路紊乱相关。Alpha-hemolysin 可用于感染性角膜炎病理机制的研究，特别是其在角膜伤口愈合延迟中的作用。阻断 Alpha-hemolysin 或限制葡萄球菌定植已成为角膜创伤和感染性疾病的潜在抑制策略。

Alpha-hemolysin Protein, S. aureus 显著抑制人角膜上皮细胞 (HCLE) 伤口，抑制角膜上皮细胞迁移^[1]。
Alpha-hemolysin Protein, S. aureus 抑制角膜上皮细胞 HCLE 细胞活力^[1]。
Alpha-hemolysin Protein, S. aureus 促进野生型菌株入侵 HCLE 细胞^[1]。

Alpha-hemolysin Protein, S. aureus (5 μg/mL，局部应用；单次) 显著延迟小鼠角膜上皮清创模型中角膜上皮伤口闭合^[1]。
Alpha-hemolysin Protein, S. aureus (3×10⁶ CFU 野生型菌株；角膜接种；单次) 增加小鼠感染性角膜炎模型的感染评分，且显著增加角膜组织内细菌载量，促进细菌在角膜的侵袭和感染^[1]。

Staphylococcus aureus

P. pastoris

N-6*His

Q2G1X0 (A27-N319)

3920722  [NCBI]

Full Length of Mature Protein

ADSDINIKTGTTDIGSNTTVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKSGLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGGLIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYGNQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYERVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN

Approximately 35 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Putra I, et al. Staphylococcus aureus alpha-hemolysin impairs corneal epithelial wound healing and promotes intracellular bacterial invasion. Exp Eye Res. 2019 Apr;181:263-270.  [Content Brief]