AMPK gamma 1/beta 2/alpha 2 Heterotrimer 蛋白, Human (sf9, His-GST)

The AMPK gamma 1 is the AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), a crucial regulator of cellular energy metabolism. Activated by low ATP levels, it enhances energy production while suppressing biosynthetic processes and cell growth. The gamma subunit mediates AMP, ADP, and ATP binding, influencing AMPK activation or inhibition. Allosteric AMP binding activates the alpha subunit, while ADP stimulates phosphorylation, and ATP promotes dephosphorylation, rendering AMPK inactive. Additionally, AMPK modulates cellular polarity and interacts with FNIP1 and FNIP2. AMPK gamma 1/beta 2/alpha 2 Heterotrimer Protein, Human (sf9, His-GST) is the recombinant human-derived AMPK gamma 1/beta 2/alpha 2 Heterotrimer protein, expressed by Sf9 insect cells, with N-His, N-GST labeled tag. AMPK gamma 1/beta 2/alpha 2 Heterotrimer Protein, Human (sf9, His-GST), has molecular weight of ~35 & 37 & 95 KDa, respectively.

AMPK γ1 是 AMP 激活蛋白激酶 (AMPK) 的 AMP/ATP 结合亚基，AMPK 是细胞能量代谢的关键调节因子。当细胞内 ATP 水平降低时，AMPK 被激活，启动一系列事件，增强能量产生，同时抑制能量消耗过程，如蛋白质、碳水化合物和脂质生物合成，以及细胞生长和增殖。AMPK 的 γ 非催化亚基介导与 AMP、ADP 和 ATP 的结合，影响 AMPK 的激活或抑制。AMP 结合通过诱导磷酸化和防止去磷酸化以变构方式激活 α 催化亚基。ADP 刺激磷酸化，而 ATP 促进去磷酸化，使 AMPK 酶失活。除了在能量调节中的作用之外，AMPK 还通过重塑肌动蛋白细胞骨架（可能通过肌球蛋白的间接激活）充当细胞极性的调节剂。AMPK 可形成异三聚体，包括 α 催化亚基（PRKAA1 或 PRKAA2）、β 亚基（PRKAB1 或 PRKAB2）和 γ 非催化亚基（PRKAG1、PRKAG2 或 PRKAG3），凸显了其复杂的调节机制。AMPK 进一步与 FNIP1 和 FNIP2 相互作用来调节其细胞功能。

Human

Sf9 insect cells

N-His;N-GST

P54619 (M1-P331)&O43741 (M1-I272)&P54646 (M1-R552)

5571  [NCBI]&5565  [NCBI]&5563  [NCBI]

Approximately 35&37&95 kDa.

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.