1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Angiopoietins
  4. Angiopoietin Like 3 Proteins
  5. ANGPTL3/Angiopoietin-like 3 蛋白, Human (Biotinylated, HEK293, His-Avi)

ANGPTL3/Angiopoietin-like 3 蛋白, Human (Biotinylated, HEK293, His-Avi)

目录号: HY-P78059
COA 产品使用指南

ANGPTL3/血管生成素样 3 蛋白是一种肝因子,复杂地调节脂质和葡萄糖代谢。它通过抑制脂蛋白脂肪酶 (LPL) 刺激血浆甘油三酯,通过 PCSK6 和 FURIN 募集抑制 TG 清除,不依赖于蛋白水解裂解,且不受 GPIHBP1 影响。ANGPTL3 抑制内皮脂肪酶,升高 HDL 胆固醇,并激活脂肪细胞脂肪分解。它选择性抑制氧化组织中的 LPL,将 VLDL-TG 导向白色脂肪组织,可能与 ANGPTL8 和 ANGPTL4 协同作用。ANGPTL3 还可以独立于经典 APOE 和 LDLR 途径降低 LDL 胆固醇,并可能刺激下丘脑 LPL 活性。ANGPTL3/Angiopoietin-like 3 蛋白, Human (Biotinylated, HEK293, His-Avi) 是重组的 ANGPTL3/血管生成素样 3 蛋白,由 HEK293 表达,带有 C-Avi, C-His 标签。ANGPTL3/Angiopoietin-like 3 蛋白, Human (Biotinylated, HEK293, His-Avi) 全长 203 个氨基酸,分子量为 30-38 kDa。

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规格 价格 是否有货 数量
20 μg ¥2800 In-stock
50 μg ¥6160 In-stock
100 μg ¥9980 询价
> 100 μg   询价  

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描述

ANGPTL3/Angiopoietin-like 3 protein, a hepatokine, intricately regulates lipid and glucose metabolism. It stimulates plasma triglycerides by inhibiting lipoprotein lipase (LPL), suppressing TG clearance through PCSK6 and FURIN recruitment, independent of proteolytic cleavage and unaffected by GPIHBP1. ANGPTL3 inhibits endothelial lipase, elevating HDL cholesterol, and activates adipocyte lipolysis. It selectively suppresses LPL in oxidative tissues, directing VLDL-TG to white adipose tissue, potentially cooperating with ANGPTL8 and ANGPTL4. ANGPTL3 also lowers LDL cholesterol independently of the canonical APOE and LDLR pathway, and may stimulate hypothalamic LPL activity. ANGPTL3/Angiopoietin-like 3 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived ANGPTL3/Angiopoietin-like 3 protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of ANGPTL3/Angiopoietin-like 3 Protein, Human (Biotinylated, HEK293, His-Avi) is 203 a.a., with molecular weight of 30-38 kDa.

研究背景

ANGPTL3/血管生成素样 3 蛋白作为肝因子发挥作用,复杂地参与脂质和葡萄糖代谢的调节。ANGPTL3 被提议在响应食物摄入而将能量底物导向储存或氧化组织方面发挥作用,它通过抑制脂蛋白脂肪酶 (LPL) 活性来刺激血浆甘油三酯 (TG),从而抑制 TG 清除。这种效果是通过将前蛋白转化酶 PCSK6 和 FURIN 募集至 LPL 来实现的,从而导致 LPL 从细胞表面裂解和解离。然而,这种调节功能不需要 ANGPTL3 蛋白水解切割,由 N 末端结构域介导,并且不受 GPIHBP1 的影响。此外,ANGPTL3 还能抑制内皮脂肪酶,从而提高血浆中高密度脂蛋白 (HDL) 胆固醇和磷脂的水平。通过与脂肪细胞结合,它激活脂肪分解,释放游离脂肪酸和甘油。ANGPTL3 选择性抑制氧化组织中的 LPL,将极低密度脂蛋白 (VLDL)-TG 引导至白色脂肪组织 (WAT) 进行储存以响应食物,可能与 WAT 中循环的肝源性 ANGPTL8 和 ANGPTL4 表达相配合。它还有助于降低血浆低密度脂蛋白 (LDL) 胆固醇水平,与经典的 APOE 和 LDLR 途径无关。此外,ANGPTL3 可能会刺激下丘脑 LPL 活性,而在体外,它会抑制 LPL 活性,但对 GPIHBP1 稳定的 LPL 没有作用。

生物活性

1. Immobilized Biotinylated Human ANGPTL3 His at 0.5 μg/mL (100 μL/Well). Dose response curve for Anti-ANGPTL3 Antibody hFc with the EC50 ≤ 11.5 ng/mL determined by ELISA.
2. Immobilized Anti-ANGPTL3 Antibody at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human ANGPTL3, His Tag with the EC50 ≤ 5.3 ng/mL determined by ELISA.

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

Q9Y5C1 (S17-K219)

基因 ID
蛋白结构
N-term
ANGPTL3 (S17-K219)
Accession # Q9Y5C1
His-Avi
C-term
同用名
ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3
分子量

30-38 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

ANGPTL3/Angiopoietin-like 3 Protein, Human (Biotinylated, HEK293, His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ANGPTL3/Angiopoietin-like 3 Protein, Human (Biotinylated, HEK293, His-Avi)
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