Animal-Free IL-10 蛋白, Human (His)

IL-10 protein is a major immunoregulatory cytokine with profound anti-inflammatory functions that limits excessive tissue destruction caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptors IL10RA and IL10RB, leading to JAK1- and STAT2-mediated phosphorylation of STAT3, which translocates to the nucleus and drives the expression of anti-inflammatory mediators. Animal-Free IL-10 Protein, Human (His) is the recombinant human-derived animal-FreeIL-10 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-10 Protein, Human (His) is 160 a.a., with molecular weight of ~19.6 kDa.

IL-10 蛋白是一种关键的免疫调节细胞因子，具有有效的抗炎作用，可防止炎症引起的过度组织损伤。它与由 IL10RA 和 IL10RB 组成的异四聚体受体结合，触发 JAK1 和 STAT2 介导的 STAT3 磷酸化。这导致 STAT3 易位到细胞核中，驱动抗炎介质的表达。IL-10 蛋白靶向巨噬细胞和单核细胞等抗原呈递细胞，抑制促炎细胞因子的释放。它还通过减少 MHC II 类和共刺激分子的表达来阻碍抗原呈递，从而抑制 T 细胞活化。此外，它还能重新编程代谢途径，包括 mTOR 信号传导，以控制巨噬细胞的炎症反应。该蛋白形成同二聚体并与 IL10RA 和 IL10RB 相互作用。

Measure by its ability to induce MC/9 - 2 cells proliferation. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant human IL-10 is approximately >1 x10⁶ IU/ mg.

Human

E. coli

C-His

P22301 (S19-N178)

3586  [NCBI]

SPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN

Approximately 19.6 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.01 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.