Animal-Free IL-10 蛋白, Mouse (His)

IL-10 protein is a key immunomodulator that, by binding to its heterotetrameric receptors (IL10RA and IL10RB), activates JAK1 and STAT2 (the latter phosphorylates STAT3), thereby triggering potent anti-inflammatory effects. Phosphorylated STAT3 translocates to the nucleus and promotes the expression of anti-inflammatory mediators. Animal-Free IL-10 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-10 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-10 Protein, Mouse (His) is 160 a.a., with molecular weight of ~19.69 kDa.

IL-10 是一种主要的免疫调节细胞因子，具有深远的抗炎功能，可减轻炎症引起的过度组织破坏。IL-10 与其异四聚体受体（包含 IL10RA 和 IL10RB）结合后，启动涉及 JAK1 和 STAT2 介导的 STAT3 磷酸化的信号级联反应。随后，磷酸化的 STAT3 易位至细胞核，驱动抗炎介质的表达。IL-10 特异性靶向抗原呈递细胞 (APC)，例如巨噬细胞和单核细胞，抑制其释放促炎细胞因子，包括 GM-CSF、G-CSF、IL-1 α、IL-1 β、IL-6 、IL-8 和 TNF-α。此外，IL-10 通过减少 MHC II 类和共刺激分子的表达来干扰抗原呈递，从而抑制它们诱导 T 细胞活化的能力。此外，IL-10 通过重新编程重要的代谢途径（包括 mTOR 信号传导）来控制巨噬细胞的炎症反应。IL-10 以同二聚体形式存在，并与 IL10RA 和 IL10RB 相互作用。

Measure by its ability to induce MC/9- 2 cells prolferation. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant mouse IL-10 is>1 x 10⁶ IU/mg.

Mouse

E. coli

C-His

P18893 (S19-S178)

16153  [NCBI]

MSRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVEQVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS

Approximately 19.69 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.