Animal-Free IL-36 alpha/IL-1F6 蛋白, Human (His)

IL-36 α/IL-1F6 protein binds to IL1RL2/IL-36R receptor, activates NF-kappa-B and MAPK pathways, and induces pro-inflammatory responses. IL-36 α/IL-1F6 also upregulates CD83, CD86, and HLA-DR in dendritic cells, promotes dendritic cell maturation, and drives T cell proliferation. Animal-Free IL-36 alpha/IL-1F6 Protein, Human (His) is the recombinant human-derived animal-FreeIL-36 alpha/IL-1F6 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-36 alpha/IL-1F6 Protein, Human (His) is 153 a.a., with molecular weight of ~18.05 kDa.

IL-36 α/IL-1F6 蛋白是一种细胞因子，与 IL1RL2/IL-36R 受体结合并通过其发出信号，激活靶细胞中的 NF-kappa-B 和 MAPK 信号通路，从而促进促炎反应。作为 IL-36 信号系统的一部分，它被认为存在于上皮屏障中并参与局部炎症反应，通过辅助受体 IL1RAP 与 IL-1 系统具有相似性。IL-36 α/IL-1F6 通过影响角质形成细胞、树突状细胞并间接影响 T 细胞、促进组织浸润、细胞成熟和细胞增殖，似乎在皮肤炎症反应中发挥着至关重要的作用。在培养的角质形成细胞中，诱导多种趋化因子和促炎细胞因子的表达，例如CCL3、CCL4、CCL5、CCL2、CCL17、CCL22、CL20、CCL5、CCL2、CCL17、CCL22、CXCL8、CCL20、CXCL1、TNF-α 、IL-8 和 IL-6。此外，IL-36 α/IL-1F6 上调培养单核细胞中 IL-1A、IL-1B 和 IL-6 的表达，促进骨髓树突状细胞的细胞成熟，并在驱动 T 细胞的同时促进树突状细胞成熟单核细胞衍生的树突状细胞的增殖。它与 TMED10 的相互作用介导从细胞质易位到内质网-高尔基中间室 (ERGIC) 以及随后的分泌。此外，IL-36 α/IL-1F6 可能有助于肺部的促炎作用。

Measure by its ability to induce IL-8 secretion in human PBMCs. The ED₅₀ for this effect is <0.7 ng/mL.

Human

E. coli

C-His

Q9UHA7 (K6-F158)

14440

MKIDTPQQGSIQDINHRVWVLQDQTLIAVPRKDRMSPVTIALISCRHVETLEKDRGNPIYLGLNGLNLCLMCAKVGDQPTLQLKEKDIMDLYNQPEPVKSFLFYHSQSGRNSTFESVAFPGWFIAVSSEGGCPLILTQELGKANTTDFGLTMLF

Approximately 18.05 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.