Animal-Free IL-36 gamma/IL-1F9 蛋白, Mouse (His)

IL-36 gamma/IL-1F9 protein activates NF-κB through IL1RL2 and promotes local inflammatory responses in the epithelial barrier. It affects keratinocytes, dendritic cells and T cells, promoting tissue infiltration, cell maturation and proliferation. Animal-Free IL-36 gamma/IL-1F9 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-36 gamma/IL-1F9 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-36 gamma/IL-1F9 Protein, Mouse (His) is 152 a.a., with molecular weight of ~18.27 kDa.

IL-36 gamma/IL-1F9 蛋白充当激动剂，通过孤儿 IL-1 受体相关蛋白 2/IL1RL2 激活 NF-κ B。作为 IL-36 信号系统的一部分，它被认为存在于上皮屏障中，有助于局部炎症反应，类似于 IL-1 系统，共享辅助受体 IL1RAP。这种蛋白质参与皮肤炎症反应，影响角质形成细胞、树突状细胞，并间接影响 T 细胞驱动组织浸润、细胞成熟和增殖。此外，它可能在特定中性粒细胞气道炎症期间的促炎反应中发挥作用，并有助于针对真菌病原体的先天免疫反应。IL-36 γ 诱导骨髓源性树突状细胞 (BMDC) 产生各种促炎细胞因子，并通过刺激 CD80、CD86 和 MHC II 类的表面表达来增强树突状细胞成熟。此外，它还能诱导培养的 CD4(+) T 细胞和脾细胞产生 IFN-γ、IL-4 和 IL-17。该蛋白与货物受体 TMED10 相互作用，介导从细胞质易位至内质网-高尔基中间室 (ERGIC) 以及随后的分泌。

Measure by its ability to induce IL-6 secretion in 3T3 cells. The ED₅₀ for this effect is <15 ng/mL. The specific activity of recombinant mouse IL-36 gamma is >6 x10⁴ IU/mg

Mouse

E. coli

C-His

Q8R460 (G13-S164)

215257  [NCBI]

MGRETPDFGEVFDLDQQVWIFRNQALVTVPRSHRVTPVSVTILPCKYPESLEQDKGIAIYLGIQNPDKCLFCKEVNGHPTLLLKEEKILDLYHHPEPMKPFLFYHTRTGGTSTFESVAFPGHYIASSKTGNPIFLTSKKGEYYNINFNLDIKS

Approximately 18.27 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.