Annexin A6/ANXA6 蛋白, Human (His)

Annexin A6 Protein, Human (His) is a Annexin A6 protein fused to His-tag at N-terminus. Annexin A6 is an endogenous and secretory multifunctional protein and locates on the cell surface. Annexin A6 can be used for the study in mediating the endosome aggregation and vesicle fusion during exocytosis^[1][2].

膜联蛋白由一个蛋白质家族组成，涉及细胞膜动力学和膜相关蛋白调节的许多方面。
膜联蛋白 6 是一种 67kDa 钙依赖性蛋白，是促进膜聚结和折叠所需的主要靶标膜修复过程中。膜联蛋白 6 参与调节细胞内储存的 Ca²⁺ 的释放，并可能与 CD21 相关。此外，膜联蛋白 6 还参与介导胞吐作用过程中分泌上皮细胞的内体聚集和囊泡融合。

Human

E. coli

C-6*His

P08133 (A2-D673)

309  [NCBI]

AKPAQGAKYRGSIHDFPGFDPNQDAEALYTAMKGFGSDKEAILDIITSRSNRQRQEVCQSYKSLYGKDLIADLKYELTGKFERLIVGLMRPPAYCDAKEIKDAISGIGTDEKCLIEILASRTNEQMHQLVAAYKDAYERDLEADIIGDTSGHFQKMLVVLLQGTREEDDVVSEDLVQQDVQDLYEAGELKWGTDEAQFIYILGNRSKQHLRLVFDEYLKTTGKPIEASIRGELSGDFEKLMLAVVKCIRSTPEYFAERLFKAMKGLGTRDNTLIRIMVSRSELDMLDIREIFRTKYEKSLYSMIKNDTSGEYKKTLLKLSGGDDDAAGQFFPEAAQVAYQMWELSAVARVELKGTVRPANDFNPDADAKALRKAMKGLGTDEDTIIDIITHRSNVQRQQIRQTFKSHFGRDLMTDLKSEISGDLARLILGLMMPPAHYDAKQLKKAMEGAGTDEKALIEILATRTNAEIRAINEAYKEDYHKSLEDALSSDTSGHFRRILISLATGHREEGGENLDQAREDAQVAAEILEIADTPSGDKTSLETRFMTILCTRSYPHLRRVFQEFIKMTNYDVEHTIKKEMSGDVRDAFVAIVQSVKNKPLFFADKLYKSMKGAGTDEKTLTRIMVSRSEIDLLNIRREFIEKYDKSLHQAIEGDTSGDFLKALLALCGGED

Approximately 60-90 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Alexis R Demonbreun, et al. Recombinant annexin A6 promotes membrane repair and protects against muscle injury. J Clin Invest. 2019 Nov 1;129(11):4657-4670.  [Content Brief]

  [2]. Ramin Raouf, et al. Inhibition of somatosensory mechanotransduction by annexin A6. Sci Signal. 2018 Jun 19;11(535):eaao2060.  [Content Brief]

  [3]. Julie Leca, et al. Cancer-associated fibroblast-derived annexin A6+ extracellular vesicles support pancreatic cancer aggressiveness. J Clin Invest. 2016 Nov 1;126(11):4140-4156.  [Content Brief]