Apolipoprotein E/APOE 蛋白, Rat (His)

Apolipoprotein E/APOE is essential for lipid transport and is integral to the production, transformation and clearance of plasma lipoproteins. It interacts with various particles to favor HDL and binds to receptors such as LDLR and VLDLR to promote cellular uptake. Apolipoprotein E/APOE Protein, Rat (His) is the recombinant rat-derived Apolipoprotein E/APOE protein, expressed by E. coli , with N-6*His labeled tag. The total length of Apolipoprotein E/APOE Protein, Rat (His) is 294 a.a., with molecular weight of 39.8 kDa.

APOE 是一种重要的蛋白质，参与通过血浆和间质液在器官之间运输脂质。它在血浆脂蛋白的产生、转化和清除中起着至关重要的作用。APOE 与各种脂蛋白颗粒相互作用，例如乳糜微粒、乳糜微粒残余物、VLDL 和 IDL，且优先考虑 HDL。它还与多种细胞受体结合，包括 LDLR 和 VLDLR，促进细胞摄取含有 APOE 的脂蛋白。此外，APOE 具有肝素结合活性，并与细胞表面的硫酸乙酰肝素蛋白聚糖结合，有助于捕获和受体介导的含 APOE 脂蛋白的摄取。此外，APOE 形成同源四聚体，并可能在 HDL 生物合成中与 ABCA1 相互作用。它还可以与脑脊液中的 APP/A4 淀粉样蛋白-β 肽、MAPT、MAP2 和分泌的 SORL1 相互作用，并与 PMEL 相互作用，诱导管腔内囊泡上的原纤维成核。

Rat

E. coli

N-6*His

P02650 (E19-Q312)

25728  [NCBI]

EGELEVTDQLPGQSDQPWEQALNRFWDYLRWVQTLSDQVQEELQSSQVTQELTVLMEDTMTEVKAYKKELEEQLGPVAEETRARLAKEVQAAQARLGADMEDLRNRLGQYRNEVNTMLGQSTEELRSRLSTHLRKMRKRLMRDADDLQKRLAVYKAGAQEGAERGVSAIRERLGPLVEQGRQRTANLGAGAAQPLRDRAQALSDRIRGRLEEVGNQARDRLEEVREQMEEVRSKMEEQTQQIRLQAEIFQARIKGWFEPLVEDMQRQWANLMEKIQASVATNSIASTTVPLENQ

45 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.