Apolipoprotein M/APOM 蛋白, Human (HEK293, His)

Apolipoprotein M Protein, Human (HEK293, His) expresses in HEK293 with a His tag at the N-terminus. Apolipoprotein E (apoE) secreted by HEK cells stably expressing apoE3 or apoE4 (HEK-apoE) binds Aβ, and inhibits Aβ-induced neurotoxicity ^[1].

载脂蛋白 E (apoE) 是一种 34 kDa 糖蛋白，是各种血浆脂蛋白颗粒的表面成分，包括乳糜微粒残留物、β-迁移 VLDL (β-VLDL)、LDL 和 HDL 亚类。在血浆胆固醇 (Chol) 和甘油三酯 (TG) 的运输和代谢过程中，ApoE 介导含 apoE 的脂蛋白与细胞表面内吞受体的高亲和力结合。HEK-apoE 是低密度脂蛋白 (LDL) 受体相关蛋白 (LRP) 的配体，但不是 LDL 受体^[1]。

Measured by its ability to bind all-trans-retinoic acid. The concentration of all-trans-retinoic acid required to quench 50% of Trp fluorescence in Recombinant Human Apolipoprotein M/ApoM is approximately 27.536 μM.

Human

HEK293

C-6*His

O95445 (C23-N188)

55937  [NCBI]

CPEHSQLTTLGVDGKEFPEVHLGQWYFIAGAAPTKEELATFDPVDNIVFNMAAGSAPMQLHLRATIRMKDGLCVPRKWIYHLTEGSTDLRTEGRPDMKTELFSSSCPGGIMLNETGQGYQRFLLYNRSPHPPEKCVEEFKSLTSCLDSKAFLLTPRNQEACELSNN

19-24 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. LaDu MJ, et al. Self-assembly of HEK cell-secreted ApoE particles resembles ApoE enrichment of lipoproteins as a ligand for the LDL receptor-related protein. Biochemistry. 2006 Jan 17;45(2):381-90.  [Content Brief]