ASAH1 蛋白, Human (His-SUMO)

ASAH1 is a lysosomal ceramidase that critically regulates sphingolipid metabolism by hydrolyzing ceramide into sphingosine and fatty acids at acidic pH. Bioactive lipids—ceramide, sphingosine, and sphingosine-1-phosphate—control signaling pathways for cell proliferation, apoptosis, and differentiation. ASAH1 Protein, Human (His-SUMO) is the recombinant human-derived ASAH1 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of ASAH1 Protein, Human (His-SUMO) is 374 a.a., with molecular weight of ~58.7 kDa.

ASAH1 是一种溶酶体神经酰胺酶，通过在酸性 pH 条件下将鞘脂神经酰胺水解为鞘氨醇和游离脂肪酸，在鞘脂代谢中发挥作用。神经酰胺、鞘氨醇和 1-磷酸鞘氨醇是生物活性脂质，在控制细胞增殖、凋亡和分化等过程的细胞信号传导途径中发挥着至关重要的作用。与其他神经酰胺相比，ASAH1 对 C12-神经酰胺表现出更高的催化效率，并且还可以催化逆反应，从脂肪酸和鞘氨醇合成神经酰胺。在该合成反应中，天然鞘氨醇D-赤式异构体得到了更有效的利用，链长为12或14个碳的脂肪酸是最有效的底物。此外，ASAH1 显示 N-酰基乙醇胺水解酶活性。通过调节神经酰胺、鞘氨醇和 1-磷酸鞘氨醇水平，ASAH1 介导钙诱导的表皮角质形成细胞分化。它间接调节肿瘤坏死因子 (TNF) 诱导的细胞凋亡，并且在肾上腺皮质细胞中，可能通过维持神经酰胺和鞘氨醇之间的细胞内平衡来调节类固醇生成。此外，ASAH1 可能通过与核受体 NR5A1 结合并负向调节其转录活性来直接影响类固醇生成。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-SUMO;N-6*His

Q13510 (22Q-395W)

427  [NCBI]

QHAPPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW

Approximately 58.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.