ATF2 蛋白, Human (sf9, His-GST)

The ATF2 protein is a transcriptional activator that regulates anti-apoptotic genes, cell growth, and DNA damage responses. It binds to CRE and AP-1 sequences and plays a key role in nuclear transcription. ATF2 Protein, Human (sf9, His-GST) is the recombinant human-derived ATF2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of ATF2 Protein, Human (sf9, His-GST) is 505 a.a., with molecular weight of ~85 kDa.

ATF2 是一种转录激活剂，协调调节参与抗凋亡、细胞生长和 DNA 损伤反应的各种基因。它具有双重 DNA 结合能力，可识别 CRE 和 AP-1 共有序列，并在细胞核内的全局转录中发挥关键作用。在细胞核外，ATF2 与线粒体外膜上含有 HK1 和 VDAC1 的复合物相互作用，破坏线粒体膜电位并诱导细胞死亡。由 ATM 介导的磷酸化形式有助于 DNA 损伤反应，参与电离辐射诱导的 S 期检查点控制并将 MRN 复合物募集到损伤位点。ATF2 还具有组蛋白乙酰转移酶 (HAT) 活性，特别是乙酰化组蛋白 H2B 和 H4。ATF2 与 CUL3 和 RBX1 合作，促进 KAT5 的降解，调节其乙酰化和激活 ATM 的能力。ATF2 表现出背景依赖性致癌或肿瘤抑制活性，与其自身或与 JUN 形成二聚体。与 SMAD3、SMAD4、UTF1、HK1/VDAC1 复合物和其他几种蛋白质的相互作用进一步凸显了 ATF2 在细胞过程中的复杂参与。

Human

Sf9 insect cells

N-His;N-GST

P15336 (M1-S505)

1386  [NCBI]

Approximately 85 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.