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  4. ATG9B 蛋白, Human (HEK293, His, MBP, FLAG)

ATG9B 蛋白, Human (HEK293, His, MBP, FLAG)

目录号: HY-P701359
产品使用指南

ATG9B 蛋白是一种对自噬至关重要的磷脂扰乱酶，在自噬体前结构 (PAS) 和细胞质囊泡池之间动态循环。它在扩张自噬体膜、促进 ATG2 脂质分布和驱动膜扩张方面发挥着关键作用。此外，ATG9B 参与坏死细胞死亡。ATG9B 蛋白, Human (HEK293, His, MBP, FLAG) 是重组的 ATG9B 蛋白，由 HEK293 表达，带有 N-MBP, C-Flag, N-8*His 标签。ATG9B 蛋白, Human (HEK293, His, MBP, FLAG) 全长 924 个氨基酸。

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描述

ATG9B Protein, a phospholipid scramblase crucial for autophagy, dynamically cycles between the preautophagosomal structure (PAS) and the cytoplasmic vesicle pool. It plays a critical role in expanding autophagosomal membranes, facilitating lipid distribution with ATG2 and driving membrane expansion. Additionally, ATG9B participates in necrotic cell death. ATG9B Protein, Human (HEK293, His, MBP, FLAG) is the recombinant human-derived ATG9B protein, expressed by HEK293 , with N-MBP, C-Flag, N-8*His labeled tag. The total length of ATG9B Protein, Human (HEK293, His, MBP, FLAG) is 924 a.a., .
研究背景

ATG9B 蛋白作为磷脂扰乱酶发挥重要作用，参与自噬，介导自噬体膜的扩张。它在自噬体前结构/噬菌体组装位点 (PAS) 和细胞质囊泡库之间动态循环，充当生长自噬体的关键膜供应商。ATG9B 的脂质扰乱酶活性在自噬体前结构/吞噬细胞组装过程中至关重要，促进通过 ATG2（ATG2A 或 ATG2B）从细胞质小叶到达双层的管腔小叶的磷脂的分布。这种活动反过来又驱动自噬体膜的扩张。除了在自噬中的作用之外，ATG9B 还参与坏死细胞死亡。
种属

Human
表达系统

HEK293
标签

N-MBP;C-Flag;N-8*His
蛋白编号

Q674R7 (M1-D924)
基因 ID

285973
蛋白结构
N-term
8*His-MBP
ATG9B (M1-D924)
Accession # Q674R7
Flag
C-term
同用名
ATG9B; Autophagy-related protein 9B; APG9-like 2; Nitric oxide synthase 3-overlapping antisense gene protein; Protein sONE
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

ATG9B Protein, Human (HEK293, His, MBP, FLAG) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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