BACE1 蛋白, Human (HEK293, N-His)

BACE1 protein is crucial in proteolytic processing of amyloid precursor protein (APP), initiating cleavage at the N-terminus of the A-beta peptide sequence. This process results in the extracellular release of beta-cleaved soluble APP and a cell-associated C-terminal fragment. BACE1's cleavage activity extends to CHL1, demonstrating its versatility in substrate processing. BACE1 Protein, Human (HEK293, N-His) is the recombinant human-derived BACE1 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of BACE1 Protein, Human (HEK293, N-His) is 436 a.a., with molecular weight of 56-85 kDa.

BACE1 蛋白在淀粉样前体蛋白 (APP) 的蛋白水解过程中发挥着关键作用。它在 A-β 肽序列的 N 末端（特别是 APP 的残基 671 和 672 之间）启动切割，从而产生 β 切割的可溶性 APP，并随后将其释放到细胞外。同时，BACE1 生成相应的细胞相关 C 末端片段，随后由 γ 分泌酶释放。除了其主要靶标外，BACE1 还对 CHL1 表现出切割活性，正如通过其切割该底物的相似性观察到的。

Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. Read at excitation and emission wavelengths of 320 nm and 405 nm. The specific activity is 173.598 pmoL/min/µg, as measured under the described conditions.

Human

HEK293

N-6*His

P56817-1 (T22-T457)

23621

TQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMT

56-85 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.