1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. BACE1 蛋白, Human (HEK293)

BACE1 蛋白, Human (HEK293)

目录号: HY-P72840
COA 产品使用指南

BACE1 蛋白是天冬氨酸蛋白酶家族的重要成员,在阿尔茨海默病病理学中至关重要。选择性剪接产生多种转录变体,其中至少一种编码前蛋白原,该前蛋白原经过蛋白水解加工产生成熟的蛋白酶。BACE1 蛋白, Human (HEK293) 是重组的 BACE1 蛋白,由 HEK293 表达,不带标签。BACE1 蛋白, Human (HEK293) 全长 436 个氨基酸,分子量约为 ~65 kDa。

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规格 价格 是否有货 数量
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5 μg ¥350 In-stock
10 μg ¥600 In-stock
50 μg ¥1650 In-stock
100 μg ¥2800 询价
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BACE1 Protein, Human (HEK293) 相关产品:

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The BACE1 protein is an important member of the aspartic protease family and is critical in Alzheimer's disease pathology. Alternative splicing generates multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to produce the mature protease. BACE1 Protein, Human (HEK293) is the recombinant human-derived BACE1 protein, expressed by HEK293 , with tag free. The total length of BACE1 Protein, Human (HEK293) is 436 a.a., with molecular weight of ~65 kDa.

研究背景

BACE1 是天冬氨酸蛋白酶肽酶 A1 家族的成员,在阿尔茨海默病病理学中发挥着关键作用。通过选择性剪接,该基因产生多种转录变体,其中至少一种编码前蛋白原,该前蛋白原经过蛋白水解加工产生成熟的蛋白酶。作为一种跨膜蛋白酶,BACE1 催化从淀粉样前体蛋白产生淀粉样β肽的初始步骤。β 淀粉样肽是阿尔茨海默病患者大脑中积累的β 淀粉样斑块的主要成分。BACE1 的表达很广泛,在多种组织中普遍存在,包括胰腺、大脑和 24 种其他组织,强调了其在不同生理环境中的重要性。

生物活性

Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. Read at excitation and emission wavelengths of 320 nm and 405 nm. The specific activity is 1.79 pmol/min/µg, as measured under the described conditions.

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P56817-1/NP_036236.1 (T22-T457)

基因 ID
蛋白结构
N-term
BACE1 (T22-T457)
Accession # NP_036236.1
C-term
中文名
重组人BACE1蛋白
同用名
Beta-secretase 1; ASP2; Memapsin-2; BACE1; BACE; KIAA1149
氨基酸序列

TQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMT

分子量

Approximately 65 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 8.0 or 50 mM Tris-HCL, 100 mM NaCl, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

BACE1 Protein, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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BACE1 Protein, Human (HEK293)
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HY-P72840
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