1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. BACE1 蛋白, Mouse (HEK293, His)

BACE1 蛋白, Mouse (HEK293, His)

目录号: HY-P72843
COA 产品使用指南

BACE1蛋白是肽酶A1家族的重要成员,参与阿尔茨海默病的发病机制。它们通过前蛋白的蛋白水解加工产生成熟的蛋白酶,催化从淀粉样前体蛋白形成淀粉样β肽的初始步骤。BACE1 蛋白, Mouse (HEK293, His) 是重组的 BACE1 蛋白,由 HEK293 表达,带有 C-His 标签。BACE1 蛋白, Mouse (HEK293, His) 全长 436 个氨基酸,分子量为 60-76 kDa。

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规格 价格 是否有货 数量
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5 μg ¥450 In-stock
10 μg ¥750 In-stock
50 μg ¥2050 In-stock
100 μg ¥3500 In-stock
500 μg ¥10500 In-stock
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

BACE1 protein is an important member of the peptidase A1 family and is involved in the pathogenesis of Alzheimer's disease. They produce mature proteases through proteolytic processing of preprotein, which catalyze the initial steps in the formation of amyloid beta peptide from the amyloid precursor protein. BACE1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived BACE1 protein, expressed by HEK293 , with C-His labeled tag. The total length of BACE1 Protein, Mouse (HEK293, His) is 436 a.a., with molecular weight of 60-76 kDa.

研究背景

BACE1 是天冬氨酸蛋白酶肽酶 A1 家族的成员,在阿尔茨海默病的发病机制中发挥着关键作用。该基因产生多种转录变体,其中一种编码前蛋白原,经过蛋白水解加工产生成熟的蛋白酶。BACE1 作为跨膜蛋白酶发挥作用,催化淀粉样前体蛋白形成淀粉样β 肽的第一步。这些肽构成了β淀粉样斑块的主要成分,该斑块在阿尔茨海默病患者的大脑中很突出。BACE1 纯合基因敲除小鼠表现出一系列神经系统缺陷、生长迟缓、代谢异常和新生儿死亡率升高。BACE1 在包括小脑和卵巢在内的各种组织中广泛表达,强调了其在中枢神经系统之外的重要性。

生物活性

Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. Read at excitation and emission wavelengths of 320 nm and 405 nm. The specific activity is 1035.061 pmoL/min/µg, as measured under the described conditions.

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

NP_035922.4 (T22-T457)

基因 ID
蛋白结构
N-term
BACE1 (T22-T457)
Accession # NP_035922.4
His
C-term
同用名
Beta-secretase 1; ASP2; Memapsin-2; BACE1; BACE; KIAA1149
氨基酸序列

THLGIRLPLRSGLAGPPLGLRLPRETDEESEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHIPNIFSLQLCGAGFPLNQTEALASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAVSQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTADMEDCGYNIPQTDESTLMT

分子量

60-76 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

BACE1 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BACE1 Protein, Mouse (HEK293, His)
目录号:
HY-P72843
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