1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. BCAT2 蛋白, Human (His, Strep)

BCAT2 蛋白, Human (His, Strep)

目录号: HY-P701340
COA 产品使用指南

各种研究表明,BCAT2 蛋白在细胞过程中发挥着关键作用,因为它催化必需支链氨基酸亮氨酸、异亮氨酸和缬氨酸分解代谢的初始反应。这种酶活性对于这些氨基酸的分解至关重要,有助于能量产生和氮平衡所需的代谢过程。BCAT2 蛋白, Human (His, Strep) 是重组的 BCAT2 蛋白,由 E. coli 表达,带有 N-Strep, N-6*His 标签。BCAT2 蛋白, Human (His, Strep) 全长 365 个氨基酸。

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规格 价格 是否有货 数量
10 μg ¥1600 In-stock
50 μg ¥4500 In-stock
100 μg   询价  

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描述

Various studies have demonstrated that the BCAT2 protein plays a critical role in cellular processes as it catalyzes the initial reactions in the catabolism of the essential branched-chain amino acids leucine, isoleucine, and valine. This enzyme activity is essential for the breakdown of these amino acids, contributing to metabolic processes necessary for energy production and nitrogen balance. BCAT2 Protein, Human (His, Strep) is the recombinant human-derived BCAT2 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of BCAT2 Protein, Human (His, Strep) is 365 a.a., .

研究背景

BCAT2(支链氨基酸转氨酶 2)在必需支链氨基酸亮氨酸、异亮氨酸和缬氨酸的分解代谢中发挥着关键作用。它催化该过程中的第一个反应,涉及这些氨基酸可逆地转氨为各自的支链 α-酮酸。这种酶活性对于调节支链氨基酸及其代谢物的水平至关重要,它们参与各种生理过程,包括能量代谢和蛋白质合成。此外,BCAT2 可以充当支链 α-酮酸的转运蛋白,有助于其细胞转运。参考文献简洁地概述了 BCAT2 在支链氨基酸分解代谢中的核心作用,并表明其可能参与相关代谢物的细胞内转运。

种属

Human

表达系统

E. coli

标签

N-Strep;N-6*His

蛋白编号

O15382 (A28-V392)

基因 ID

587

蛋白结构
N-term
Strep-6*His
BCAT2 (A28-V392)
Accession # O15382
C-term
同用名
BCAT2; Branched-chain-amino-acid aminotransferase; mitochondrial; BCAT(m); Placental protein 18; PP18
分子量

Approximately 44.4 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

溶液

组分

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

Please use rapid thawing with running water to thaw the protein.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

BCAT2 Protein, Human (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BCAT2 Protein, Human (His, Strep)
目录号:
HY-P701340
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