1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TGF-beta Superfamily
  4. Bone Morphogenetic Proteins (BMPs)
  5. BMP-4
  6. BMP-4 蛋白, Mouse (HEK293, Fc)

BMP-4 蛋白, Mouse (HEK293, Fc)

目录号: HY-P74379
COA 产品使用指南

骨形态发生蛋白 4 (BMP-4) 是一种多效性配体蛋白,属于 TGFβ 家族,参与血管系统循环,可以激活血管细胞上的受体。BMP-4 结合 I 型受体 (ALK-2/-3/-6) 和 II 型受体 (BMPR2, ACVR2A),通过其促炎和促动脉粥样硬化作用增加斑块形成,促进氧化应激、内皮功能障碍和成骨分化。重组小鼠 BMP-4 蛋白 (HEK293, Fc) 全长 116 个氨基酸 (S293-R408),由 HEK293 细胞表达,带有 N 端 rFc 标签。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
5 μg ¥1170 In-stock
10 μg ¥2000 In-stock
50 μg ¥5400 In-stock
100 μg   询价  

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描述

Bone morphogenetic protein 4 (BMP-4) is a polymorphic ligand protein belonging to the TGF-β family, which is involved in the circulation of the vascular system and can activate receptors on vascular cells[1]. BMP-4 binds to type I receptors (ALK-2/-3/-6) and type II receptors (BMPR2, ACVR2A)[2] to increase plaque formation and promote oxidative stress, endothelial dysfunction, and osteogenic differentiation through its pro-inflammatory and pro-atherogenic effects[3]. BMP-4 Protein, Mouse (HEK293, Fc) has a total length of 116 amino acids (S293-R408), is expressed in HEK293 cells with N-terminal rFc-tag.

研究背景

骨形态发生蛋白4(BMP-4)是一种多效性配体蛋白,属于TGFβ家族。BMP-4 参与脉管系统循环,可激活血管细胞上的受体[1]
BMP-4/TGFβ 信号传导可被抑制性 SMAD 终止,包括 SMAD6 和 SMAD7,这些 SMAD 被激活BMP-4 广泛存在于不同动物体内,而人类的序列与大鼠高度相似(96.81 %) 和小鼠 (97.54%)。
BMP-4 由内皮细胞 (EC) 在缺氧反应中表达,并促进血管 SMC 增殖。因此,它抑制从近端肺动脉分离的平滑肌细胞 (SMC) 增殖,同时诱导从远端肺动脉分离的 SMC 增殖[5]
BMP-4 似乎是一种血管钙化的标志物和驱动因素,特别是在动脉粥样硬化中[6]
BMP-4 诱导血管生成、内皮细胞 (EC) 增殖和迁移[7] .
BMP-4 在钙化动脉粥样硬化斑块中差异表达[8],作为动脉粥样硬化血管钙化与正常骨形成机制之间的链接器[9] .
BMP-4 通过其促炎和促动脉粥样硬化作用增加斑块形成,促进氧化应激、内皮功能障碍和成骨分化[3]

体外研究
(In Vitro)

BMP-4 (1 ng/mL; 2-4 h) inhibits ERK activity in mouse embryonic stem cell (ESC) via dual-specificity phosphatase 9[1].

种属

Mouse

表达系统

HEK293

标签

N-rFc

蛋白编号

P21275 (S293-R408)

基因 ID

12159  [NCBI]

蛋白结构
N-term
rFc
BMP-4 (S293-R408)
Accession # P21275
C-term
中文名
重组小鼠 BMP-4 蛋白 (HEK293, Fc)
同用名
BMP-2B; BMP-4; Bone morphogenetic protein 4; DVR4
分子量

Approximately 40.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BMP-4 Protein, Mouse (HEK293, Fc)
目录号:
HY-P74379
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