Brorin/VWC2 蛋白, Human (HEK293, His)

The Brorin/VWC2 protein is a bone morphogenetic protein antagonist that promotes cell adhesion and associates peripherally with the AMPAR complex, a key player in synaptic transmission. In the AMPAR complex, Brorin/VWC2 and various proteins (such as CNIH2, CNIH3, CACNG2, etc.) together form a complex structure. Brorin/VWC2 Protein, Human (HEK293, His) is the recombinant human-derived Brorin/VWC2 protein, expressed by HEK293 , with C-His labeled tag. The total length of Brorin/VWC2 Protein, Human (HEK293, His) is 325 a.a., with molecular weight of ~44 kDa.

Brorin/VWC2 蛋白是一种骨形态发生蛋白 (BMP) 拮抗剂，参与神经发育并充当细胞粘附促进剂。它与 α-氨基-3-羟基-5-甲基-4-异恶唑丙酸受体 (AMPAR) 复合物外周相关，后者是突触传递的关键参与者。AMPAR 复合体由内核组成，内核包含成孔 GluA/GRIA 蛋白和以双重对称排列的主要辅助亚基。Brorin/VWC2 作为外围相关成分之一，有助于形成 AMPAR 复合体的复杂结构。该复合物包括多种蛋白质，如 CNIH2、CNIH3、CACNG2、CACNG3、CACNG4、CACNG8、GSG1L、PRRT1、PRRT2、CKAMP44/SHISA9、FRRS1L 和 NRN1 等。这些蛋白质共同形成一个平台，调节 AMPAR 复合物的门控、药理学、生物发生和蛋白质加工，从而影响突触功能和可塑性。

Human

HEK293

C-6*His

Q2TAL6 (S28-M325)

375567  [NCBI]

SPSIPLEKLAQAPEQPGQEKREHASRDGPGRVNELGRPARDEGGSGRDWKSKSGRGLAGREPWSKLKQAWVSQGGGAKAGDLQVRPRGDTPQAEALAAAAQDAIGPELAPTPEPPEEYVYPDYRGKGCVDESGFVYAIGEKFAPGPSACPCLCTEEGPLCAQPECPRLHPRCIHVDTSQCCPQCKERKNYCEFRGKTYQTLEEFVVSPCERCRCEANGEVLCTVSACPQTECVDPVYEPDQCCPICKNGPNCFAETAVIPAGREVKTDECTICHCTYEEGTWRIERQAMCTRHECRQM

Approximately 44 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.