BTLA/CD272 蛋白, Human (Biotinylated, HEK293, His-Avi)

The BTLA/CD272 protein is expressed on lymphocytes and is a negative regulator of antigen receptor signaling. It interacts with the tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 to regulate immune responses and maintain lymphocyte homeostasis. BTLA/CD272 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived BTLA/CD272 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of BTLA/CD272 Protein, Human (Biotinylated, HEK293, His-Avi) is 120 a.a., with molecular weight of 38-50 kDa.

BTLA/CD272 是淋巴细胞上表达的抑制性受体，通过与酪氨酸磷酸酶 PTPN6/SHP-1 和 PTPN11/SHP-2 相互作用，充当抗原受体信号传导的负调节因子。这些相互作用有助于调节免疫反应和维持淋巴细胞稳态。BTLA 可能与 TNFRSF14 发生顺式和反式相互作用，顺式相互作用在幼稚 T 细胞中发挥调节作用，抑制反式相互作用以维持静息状态。相比之下，在适应性免疫反应期间占主导地位的反式相互作用为效应 T 细胞提供生存信号。BTLA 与其结合伙伴之间错综复杂的相互作用强调了其在免疫调节中的多方面作用。

Measured by its binding ability in a functional ELISA. When immobilized Anti-BTLA Antibody,hFc Tag at 0.5μg/ml (100μl/well), can bind Biotinylaterd Human BTLA, His Tag with the EC50 of <10 ng/ml.

Human

HEK293

C-Avi;C-His

Q7Z6A9 (K31-S150)

151888  [NCBI]

38-50 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.