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  3. C-Reactive 蛋白, Human (His)

C-Reactive 蛋白, Human (His)

目录号: HY-P7874
COA 产品使用指南

反应蛋白是一种急性时相蛋白,是炎症的标志物,由肝脏产生。它被用作评估各种疾病中炎症的存在和严重程度的诊断工具。C-反应蛋白在炎症检测中的作用及其在疾病管理中的临床意义使其成为医学研究的热点。C-Reactive 蛋白, Human (His) 是重组的 C 反应蛋白,由 E. coli 表达,带有 C-6*His 标签。C-Reactive 蛋白, Human (His) 全长 206 个氨基酸,分子量约为 ~27.0 kDa。

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规格 价格 是否有货 数量
10 μg ¥500 In-stock
50 μg ¥1500 In-stock
100 μg   询价  

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描述

C-Reactive Protein, an acute-phase protein, is a marker of inflammation and is produced by the liver. It is used as a diagnostic tool to assess the presence and severity of inflammation in various diseases. C-Reactive Protein's role in inflammation detection and its clinical significance in disease management make it a subject of interest in medical research. C-Reactive Protein, Human (His) is the recombinant human-derived C-Reactive protein, expressed by E. coli , with C-6*His labeled tag. The total length of C-Reactive Protein, Human (His) is 206 a.a., with molecular weight of ~27.0 kDa.

研究背景

C反应蛋白(CRP)表现出与宿主防御相关的各种功能。它以钙依赖性方式与磷酸胆碱结合,促进凝集、细菌荚膜肿胀、吞噬作用和补体固定。CRP 还能够与 DNA 和组蛋白相互作用,并且可以清除受损循环细胞释放的核物质。

种属

Human

表达系统

E. coli

标签

C-6*His

蛋白编号

P02741 (Q19-P224)

基因 ID
蛋白结构
N-term
C-Reactive (Q19-P224)
Accession # P02741
6*His
C-term
同用名
rHuC-reactive protein/CRP, His; C-Reactive Protein; CRP; PTX1
氨基酸序列

QTDMSRKAFVFPKESDTSYVSLKAPLTKPLKAFTVCLHFYTELSSTRGYSIFSYATKRQDNEILIFWSKDIGYSFTVGGSEILFEVPEVTVAPVHICTSWESASGIVEFWVDGKPRVRKSLKKGYTVGAEASIILGQEQDSFGGNFEGSQSLVGDIGNVNMWDFVLSPDEINTIYLGGPFSPNVLNWRALKYEVQGEVFTKPQLWP

分子量

Approximately 27.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 2 M Urea, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

C-Reactive Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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