CA5A 蛋白, Human (His-Met)

CA5A protein is a mitochondrial carbonic anhydrase that catalyzes the reversible conversion of carbon dioxide to bicarbonate/HCO3, providing essential bicarbonate for mitochondrial enzymes. Impermeable mitochondria rely on CA5A to support enzymatic reactions critical to intermediary metabolism, including the urea cycle and the Krebs cycle. CA5A Protein, Human (His-Met) is the recombinant human-derived CA5A protein, expressed by E. coli , with N-Met, C-His labeled tag. The total length of CA5A Protein, Human (His-Met) is 266 a.a., with molecular weight of ~33 kDa.

CA5A 蛋白是一种线粒体碳酸酐酶，对于线粒体内二氧化碳可逆转化为碳酸氢盐/HCO3 至关重要，其催化活性表明。鉴于线粒体不能渗透 HCO3，CA5A 在为各种线粒体酶提供碳酸氢盐方面发挥着关键作用。这些酶是中间代谢中必需代谢物合成的组成部分，特别是在尿素循环和克雷布斯循环等过程中。CA5A 的活性强调了其通过促进关键酶反应中碳酸氢盐的可用性来维持线粒体功能代谢完整性的重要性。

Measured by its esterase activity. The specific activity is >500 pmoles/min/μg.

Human

E. coli

C-His

P35218 (A40-S305)

763  [NCBI]

Approximately 33 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM NaAc, 50 mM NaCl, 0.05% Brij 35, pH 5.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.