Carboxylesterase 1 蛋白, Human (HEK293, His)

Carboxylesterase 1 Protein, Human (HEK293, His) is an approximately 60.0 kDa human carboxylesterase 1 protein with a His-flag. Human Carboxylesterase 1 is the key liver microsomal enzyme that takes part in detoxification and metabolism^[1]. 

羧酸酯酶是作用于多种外源（例如可卡因）和内源（例如酰基辅酶A酯）底物的酶家族。羧酸酯酶由羧酸酯酶（CES 或 Ces）基因^[1]编码。
羧酸酯酶被定义为水解酯、酰胺或硫酯与其相应的醇、胺或硫醇和游离酸键合。人羧酸酯酶 1 (hCES1) 是负责多种药物解毒和代谢的关键肝微粒体酶^[1]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P23141-3 (H19-H564)

1066  [NCBI]

HPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTPPQPAEPWSFVKNATSYPPMCTQDPKAGQLLSELFTNRKENIPLKLSEDCLYLNIYTPADLTKKNRLPVMVWIHGGGLMVGAASTYDGLALAAHENVVVVTIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIASFGGNPGSVTIFGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQIAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQPLLGTVIDGMLLLKTPEELQAERNFHTVPYMVGINKQEFGWLIPMLMSYPLSEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFLDLIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDHGDELFSVFGAPFLKEGASEEEIRLSKMVMKFWANFARNGNPNGEGLPHWPEYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEHHHHHH

Approximately 60.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM HAc-NaAc, 150 mM NaCl, pH 4.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Victoria Arena de Souza, et al. Comparison of the Structure and Activity of Glycosylated and Aglycosylated Human Carboxylesterase 1. PLoS One  [Content Brief]