Carboxypeptidase A2/CPA2 蛋白, Rat (HEK293, His)

Carboxypeptidase A2/CPA2 protein is a member of the key peptidase M14 family, which plays an important role in peptide hydrolysis and contributes to the regulation of biological pathways. CPA2 may share conserved features with related proteins. Carboxypeptidase A2/CPA2 Protein, Rat (HEK293, His) is the recombinant rat-derived Carboxypeptidase A2/CPA2 protein, expressed by HEK293 , with C-His labeled tag. The total length of Carboxypeptidase A2/CPA2 Protein, Rat (HEK293, His) is 417 a.a., with molecular weight of ~45 kDa.

羧肽酶 A2/CPA2 蛋白是肽酶 M14 家族的关键成员，表明其作为肽酶的重要作用。作为该酶家族的一部分，CPA2 可能与相关蛋白质具有保守的结构和功能特征，这表明它参与肽键的水解。它属于肽酶 M14 家族，强调了它在与肽裂解和各种生物途径调节相关的细胞过程中的重要性。CPA2 的研究深入了解了其在肽酶 M14 家族背景下的特定酶功能，提供了在治疗干预中的潜在应用，并更深入地了解了其对涉及肽代谢的细胞过程的更广泛影响。对 CPA2 作用的进一步探索有望增强我们对其对正常生理和病理状况的贡献的理解。

Measured by its ability to cleave the 200 μM colorimetric peptide substrate Ac-Phe-Thiaphe-OH, in the presence of 200 μM 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB) that incubate at room temperature for 5 minutes. The specific activity is 4697.712 pmol/min/µg.

Rat

HEK293

C-10*His

G3V976 (M1-Y417)

296959  [NCBI]

MRLTLLLAALLGYIYCQETFVGDQVLEIIPSHEEQIRTLLQLEAEEHLELDFWKSPTIPGETVHVRVPFASIQAVKVFLESQGIDYSIMIEDVQVLLDQEREEMLFNQQRERGGNFNFEAYHTLEEIYQEMDNLVAENPGLVSKVNIGSSFENRPMNVLKFSTGGDKPAIWLDAGIHAREWVTQATALWTANKIASDYGTDPAITSLLNTLDIFLLPVTNPDGYVFSQTTNRMWRKTRSKRSGSGCVGVDPNRNWDANFGGPGASSSPCSDSYHGPKPNSEVEVKSIVDFIKSHGKVKAFITLHSYSQLLMFPYGYKCTKPDDFNELDEVAQKAAQALKRLHGTSYKVGPICSVIYQASGGSIDWAYDLGIKYSFAFELRDTGFYGFLLPAKQILPTAEETWLGLKTIMEHVRDHPY

Approximately 45 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.