1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) Cathepsin
  4. Cathepsin K
  5. Cathepsin K 蛋白, Mouse (His)

Cathepsin K 蛋白, Mouse (His)

目录号: HY-P72157
COA 产品使用指南

组织蛋白酶 K 蛋白是一种硫醇蛋白酶,在破骨细胞骨吸收中至关重要,并在酸性条件下表现出针对纤维蛋白原的有效内切蛋白酶活性。它在细胞外基质降解中的潜在作用值得注意。Cathepsin K 蛋白, Mouse (His) 是重组的 组织蛋白酶 K 蛋白,由 E. coli 表达,带有 N-6*His 标签。Cathepsin K 蛋白, Mouse (His) 全长 215 个氨基酸,分子量约为 ~31 kDa。

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规格 价格 是否有货 数量
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5 μg ¥1100 In-stock
10 μg ¥1870 In-stock
20 μg ¥3180 In-stock
50 μg ¥5750 In-stock
100 μg ¥8900 询价
> 100 μg   询价  

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描述

Cathepsin K protein is a thiol protease that is critical in osteoclastic bone resorption and exhibits potent endoprotease activity against fibrinogen under acidic conditions. Its potential role in extracellular matrix degradation is noteworthy. Cathepsin K Protein, Mouse (His) is the recombinant mouse-derived Cathepsin K protein, expressed by E. coli , with N-6*His labeled tag. The total length of Cathepsin K Protein, Mouse (His) is 215 a.a., with molecular weight of ~31 kDa.

研究背景

组织蛋白酶 K 蛋白是一种硫醇蛋白酶,是破骨细胞骨吸收的组成部分,在酸性条件下表现出针对纤维蛋白原的有效内切蛋白酶活性。此外,它可能在细胞外基质降解中发挥重要作用(通过相似性)。值得注意的是,研究表明,组织蛋白酶 K 通过甲状腺滤泡腔中 TG/甲状腺球蛋白的有限蛋白水解参与甲状腺激素甲状腺素 (T4) 的释放。这种双重功能强调了它在骨代谢和细胞外基质动力学中的重要性,以及它在甲状腺激素调节释放中的具体参与。

生物活性

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Measured by its ability to cleave the fluorogenic peptide substrate, Z-Gly-Pro-Arg-MCA.The specific activity is 1949.534 pmol/min/µg, as measured under the described conditions.

种属

Mouse

表达系统

E. coli

标签

N-6*His

蛋白编号

P55097 (V115-M329)

基因 ID
蛋白结构
N-term
6*His
Cathepsin K (V115-M329)
Accession # P55097
C-term
同用名
Ctsk; Cathepsin K; EC 3.4.22.38
氨基酸序列

VPDSIDYRKKGYVTPVKNQGQCGSCWAFSSAGALEGQLKKKTGKLLALSPQNLVDCVTENYGCGGGYMTTAFQYVQQNGGIDSEDAYPYVGQDESCMYNATAKAAKCRGYREIPVGNEKALKRAVARVGPISVSIDASLASFQFYSRGVYYDENCDRDNVNHAVLVVGYGTQKGSKHWIIKNSWGESWGNKGYALLARNKNNACGITNMASFPKM

分子量

Approximately 31 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Cathepsin K Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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