CCL27 蛋白, Human

CCL27 Protein, Human is a CC chemokine that binds to the chemokine receptor CCR10, attracts skin-associated memory T lymphocytes, mediates lymphocyte homing to skin sites, and plays a key role in skin inflammation. CCL27 Protein, Human a recombinant human CCL27(F25-G112) protein expressed by E. coli^[1][2].

CCL27，也称为皮肤T细胞吸引趋化因子（CTACK），是一种CC趋化因子，位于人类基因组中的第9号染色体上。CCL27在基底角蛋白形成细胞中大量表达。在正常皮肤的基底上基底角蛋白形成细胞中表达最低；然而，它们在人类 AD、接触性皮炎和牛皮癣的病变中表现出更强的 CCL27 表达。此外，CCL27存在于表皮丛、成纤维细胞和内皮细胞的真皮细胞外基质（ECM）中，尤其是在发炎的皮肤中。通过与趋化因子受体 10 (CCR10) 结合，CCL27 通过促进淋巴细胞迁移到皮肤中来调节炎症。CCL27 通过表现出高受体和组织特异性来促进组织限制性白细胞运输，并通过促进淋巴细胞迁移到皮肤中来诱导炎症。CCL27 通过促炎因子 TNFα 和 IL-1 诱导培养的正常人角质形成细胞 (NHEK) 的表达^[1][2]。

CCL27(human, intradermal injection, 0.2, 2.0 or 20 µg) induces the recruitment of lymphocytes and increases T lymphocytes as well as CD3+ lymphocytes in BALB/c mice^[3].

Human

E. coli

Tag Free

Q9Y4X3 (F25-G112)

10850  [NCBI]

FLLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQRSICIHPQNPSLSQWFEHQERKLHGTLPKLNFGMLRKMG

Approximately 14 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM PB, 500 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Lin Chen, et al. CCL27 is a critical factor for the development of atopic dermatitis in the keratin-14 IL-4 transgenic mouse model. Int Immunol. 2006 Aug;18(8):1233-42.  [Content Brief]

  [2]. Jette Lindorff Riis, et al. CCL27 expression is regulated by both p38 MAPK and IKKβ signalling pathways. Cytokine. 2011 Dec;56(3):699-707.  [Content Brief]

  [3]. Bernhard Homey, et al. CCL27-CCR10 interactions regulate T cell-mediated skin inflammation. Nat Med. 2002 Feb;8(2):157-65.  [Content Brief]