CCN1/Cyr61 蛋白, Mouse (His)

CCN1/Cyr61 Protein, Mouse (His) is the recombinant mouse-derived CCN1/Cyr61 protein, expressed by E. coli, with N-10*His tag.

CCN1/Cyr61 蛋白在细胞过程中发挥多方面的作用，积极促进细胞增殖、趋化性、血管生成和粘附。它通过协调与皮肤成纤维细胞中血管生成、炎症和基质重塑相关的关键基因的上调，证明了对伤口愈合的关键参与。CCN1 的基因调节作用依赖于肝素结合。此外，它还通过下调 1 型胶原蛋白亚基 alpha-1 和 alpha-2 的表达来发挥调节控制作用。该蛋白通过整合素 α-6/β-1 促进细胞粘附和信号传导，通过整合素 α-v/β-5 促进细胞迁移，并通过整合素 α-v/β-3 刺激细胞增殖。它与整合素的相互作用是上下文相关的，依赖于肝素和特定的细胞类型。在皮肤成纤维细胞中，CCN1 结合 ITGA6/ITGB1，在内皮细胞中，它结合 ITGAV/ITGB3，在血小板中，它与 ITGA2B/ITGB3 结合，从而影响多种细胞功能。

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Recombinant Human Cyr61/CCN1 Fc Chimera immobilized at 1.5 μg/mL, 100 μL/well, will mediate >25 % BALB/3T3 cell adhesion (3 x 10⁴ cell/well added).

Mouse

E. coli

N-10*His

P18406 (T25-D379)

16007

Full Length of Mature Protein

TCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQLNEDCSKTQPCDHTKGLECNFGASSTALKGICRAQSEGRPCEYNSRIYQNGESFQPNCKHQCTCIDGAVGCIPLCPQELSLPNLGCPNPRLVKVSGQCCEEWVCDEDSIKDSLDDQDDLLGLDASEVELTRNNELIAIGKGSSLKRLPVFGTEPRVLFNPLHAHGQKCIVQTTSWSQCSKSCGTGISTRVTNDNPECRLVKETRICEVRPCGQPVYSSLKKGKKCSKTKKSPEPVRFTYAGCSSVKKYRPKYCGSCVDGRCCTPLQTRTVKMRFRCEDGEMFSKNVMMIQSCKCNYNCPHPNEASFRLYSLFNDIHKFRD

Approximately 44 kDa, based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.