1. 重组蛋白
  2. Cytokines and Growth Factors Receptor Proteins
  3. Chemokine & Receptors G-Protein-Coupled Receptors (GPCRs)
  4. CC Chemokine Receptor Chemokine Receptor
  5. CCR9
  6. CCR9 Protein-VLP, Human (HEK293)

CCR9 Protein-VLP, Human (HEK293)

目录号: HY-P704439
产品使用指南 技术支持

CCR9 蛋白是 SCYA25/TECK 的受体,可激活信号传导,提高细胞内钙离子的浓度。在微生物感染中,它与 CD4 一起充当替代 HIV-1 辅助受体,影响感染过程。CCR9 Protein-VLP, Human (HEK293) 是重组的 CCR9 蛋白,由 HEK293 表达,不带标签。VLPs在HEK293细胞培养中形成,将膜蛋白集中在细胞表面,产生适合免疫和抗体筛选的高浓度蛋白。VLPs免疫原性强,需优化免疫策略,包括抗原剂量、接种方案和佐剂选择。本产品不推荐应用于受配体互作实验和SPR/BLI分析, 因为VLP包膜上还有宿主其他无关膜蛋白, 受配体互作会有很强的背景干扰。SPR/BLI分析则对芯片和实验方案有较高要求,成功率较低。如果需要VLP对照,推荐HY-P705433。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
20 μg ¥7350 1 - 2 周
100 μg ¥14700 1 - 2 周
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

CCR9 Protein, a receptor for SCYA25/TECK, activates signaling, elevating intracellular calcium ions. In microbial infection, it acts as an alternative HIV-1 coreceptor with CD4, influencing the infection process. CCR9 Protein-VLP, Human (HEK293) is the recombinant human-derived CCR9 protein, expressed by HEK293, with tag free. Formed in HEK293 cultures, these VLPs concentrate membrane proteins on the cell surface, producing high-concentration proteins ideal for immunization and antibody screening. Given the high immunogenicity of VLPs, the immunization protocol such as antigen dose, schedule, and adjuvant, requires optimization. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P705433.

研究背景

CCR9 蛋白作为趋化因子 SCYA25/TECK 的受体发挥作用,导致信号转导,增加细胞内钙离子水平。在微生物感染的情况下,它作为 HIV-1CD4 的替代辅助受体,促进感染过程。

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P51686-1 (T2-S48)

基因 ID

10803

蛋白结构
N-term
CCR9 (T2-S48)
Accession # P51686-1
C-term
蛋白长度

Full Length

同用名
chemokine (C-C motif) receptor 9; GPR28; C-C chemokine receptor type 9; CDw199; GPR 9 6; G protein-coupled receptor 28; GPR-9-6; CC-CKR-9;
糖基化修饰
Yes
性状

溶液.

组分

Supplied as 0.2 μm filtered solution of PBS, Arginine, pH7.4 with trehalose as protectant.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CCR9 Protein-VLP, Human (HEK293)
目录号:
HY-P704439
需求量: