1. 重组蛋白
  2. CD Antigens
  3. Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins
  4. CD14 B7-H3/CD276
  5. CD14 蛋白, Cynomolgus (HEK293, His)

CD14 蛋白, Cynomolgus (HEK293, His)

目录号: HY-P75445
产品使用指南

CD14蛋白是关键的辅助受体,与LBP配合结合LPS并将其递送至LY96/TLR4复合物以产生免疫反应。CD14 通过 MyD88、TIRAP 和 TRAF6 激活 NF-kappa-B,引发炎症和细胞因子释放。CD14 蛋白, Cynomolgus (HEK293, His) 是重组的 CD14 蛋白,由 HEK293 表达,带有 C-His 标签。CD14 蛋白, Cynomolgus (HEK293, His) 全长 344 个氨基酸,分子量为 44-48 kDa。

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描述

CD14 protein is a key coreceptor that cooperates with LBP to bind LPS and deliver it to the LY96/TLR4 complex to generate an immune response. CD14 activates NF-kappa-B through MyD88, TIRAP and TRAF6, triggering inflammation and cytokine release. CD14 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived CD14 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD14 Protein, Cynomolgus (HEK293, His) is 344 a.a., with molecular weight of 44-48 kDa.

研究背景

CD14 蛋白是细菌脂多糖 (LPS) 的关键共受体,与 LBP 合作结合单体 LPS 并将其递送至 LY96/TLR4 复合物,从而协调先天免疫反应。CD14 通过 MyD88、TIRAP 和 TRAF6 发挥作用,激活 NF-kappa-B,引发细胞因子释放和炎症。它还充当 TLR2:TLR6 和 TLR2:TLR1 异二聚体的共受体,分别响应二酰化和三酰化脂肽。此外,CD14 结合负电性 LDL,介导 LDL(-) 诱导的细胞因子释放。作为脂多糖 (LPS) 受体复合体(包括 CD14、LY96 和 TLR4)的成员,CD14LPAR1 相互作用,突出了其在免疫和信号传导通路中的多功能作用。

种属

Cynomolgus

表达系统

HEK293

标签

C-His

蛋白编号

B3Y6B8 (M1-M344)

基因 ID

697482  [NCBI]

蛋白结构
N-term
CD14 (M1-M344)
Accession # B3Y6B8
His
C-term
同用名
Monocyte Differentiation Antigen CD14; CD14
分子量

44-48 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD14 Protein, Cynomolgus (HEK293, His)
目录号:
HY-P75445
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