1. 重组蛋白
  2. Immune Checkpoint Proteins CD Antigens
  3. Stimulatory Immune Checkpoint Molecules T Cell CD Proteins B Cell CD Proteins NK Cell CD Proteins Macrophage CD Proteins Dendritic Cell CD Proteins
  4. CD28
  5. CD28 蛋白, Human/Cynomolgus (HEK293, His)

CD28 蛋白, Human/Cynomolgus (HEK293, His)

目录号: HY-P70486
COA 产品使用指南

CD28 蛋白对于 T 细胞激活、增强增殖、细胞因子产生和存活至关重要。当与 TCR/CD3 和 CD40L 连接时,CD28 促进 IL4 和 IL10 的产生,从而调节免疫反应。CD28 蛋白, Human/Cynomolgus (HEK293, His) 是重组的 CD28 蛋白,由 HEK293 表达,带有 N-6*His 标签。CD28 蛋白, Human/Cynomolgus (HEK293, His) 全长 134 个氨基酸,分子量为 30-40 kDa。

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规格 价格 是否有货 数量
10 μg ¥800 In-stock
50 μg ¥2400 In-stock
100 μg   询价  

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描述

The CD28 protein is critical for T cell activation, enhancing proliferation, cytokine production, and survival. When linked to TCR/CD3 and CD40L, CD28 promotes the production of IL4 and IL10, thereby regulating immune responses. CD28 Protein, Human/Cynomolgus (HEK293, His) is the recombinant human-derived CD28 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of CD28 Protein, Human/Cynomolgus (HEK293, His) is 134 a.a., with molecular weight of 30-40 kDa.

研究背景

CD28 蛋白在 T 细胞激活、促进细胞增殖、细胞因子产生和 T 细胞存活中发挥着关键作用。与 TCR/CD3 和 CD40L 共刺激连接后,CD28 增强 T 细胞中 IL4 和 IL10 的产生,有助于免疫反应调节。此外,CD28 的异构体 3 促进 T 细胞中 CD40L 介导的 NF-κ-B 以及激酶 MAPK8 和 PAK2 的激活。该蛋白通过二硫键形成同型二聚体,并与多种分子相互作用,包括 DUSP14、CD80/B7-1、CD86/B7-2/B70 和 GRB2。同工型 3 与 CD40LG 特异性相互作用,突出了其在介导免疫反应和细胞信号传导途径中的多方面作用。

种属

Human

表达系统

HEK293

标签

N-6*His

蛋白编号

P10747 (N19-P152)

基因 ID

940  [NCBI]

蛋白结构
N-term
6*His
CD28 (N19-P152)
Accession # P10747
C-term
同用名
CD28; CD28 antigen; CD28 molecule; T-cell-specific surface glycoprotein CD28; Tp44; TP44
氨基酸序列

NKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKP

分子量

30-40 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD28 Protein, Human/Cynomolgus (HEK293, His)
目录号:
HY-P70486
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