CD3 zeta/CD247 蛋白, Human (His)

CD3 zeta/CD247 is a component of the TCR-CD3 complex and can transmit APC-induced TCR signals to initiate immune responses. CD3D, CD3E, CD3G, and CD3Z contain ITAMs that are phosphorylated by LCK and FYN upon TCR engagement, providing docking sites for ZAP70. CD3 zeta/CD247 Protein, Human (GST) is the recombinant human-derived CD3 zeta/CD247 protein, expressed by E. coli , with N-GST labeled tag. The total length of CD3 zeta/CD247 Protein, Human (GST) is 113 a.a., with molecular weight of 28-43 KDa.

CD3 zeta/CD247 是 T 淋巴细胞表面 TCR-CD3 复合物的组成部分，在适应性免疫反应中发挥着至关重要的作用。当被抗原呈递细胞 (APC) 激活时，T 细胞受体 (TCR) 介导的信号通过 CD3 链（包括 CD3D、CD3E、CD3G 和 CD3Z）跨细胞膜传递。这些链的胞质结构域中含有基于免疫受体酪氨酸的激活基序 (ITAM)，一旦与 TCR 结合，就会被 Src 家族蛋白酪氨酸激酶 LCK 和 FYN 磷酸化。CD3Z ITAM 的磷酸化为蛋白激酶 ZAP70 创建多个对接位点，导致 ZAP70 磷酸化并转化为催化活性酶。除了在信号转导中的作用外，CD3Z 对于胸腺内 T 细胞分化也至关重要，并有助于视网膜神经节细胞 (RGC) 和背外侧膝状核 (dLGN) 的活动依赖性突触形成。TCR-CD3复合物包含异二聚体CD3D/CD3E和CD3G/CD3E，优先与TCRα和TCRβ缔合，形成三聚体。该六聚体与 CD3Z 同二聚体相互作用，形成 TCR-CD3 复合物，证明了其在 TCR 组装中的多功能性。此外，CD3Z 与多种蛋白质相互作用，例如 SLA、TRAT1、DOCK2、SHB、ZAP70 等，有助于其在免疫反应和细胞相互作用中发挥多方面的功能。

Measured by its ability to inhibit the cell growth of Jurkat cells. The ED₅₀ for this effect is 198.5 ng/mL, corresponding to a specific activity is 5.038×10³ units/mg.

Human

E. coli

N-His

P20963-1 (R52-R164)

919  [NCBI]

RVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPQRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR

Approximately 16 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.