1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens
  3. TNF Superfamily T Cell CD Proteins Macrophage CD Proteins
  4. TNF Superfamily Ligands CD153/CD30L
  5. CD30 Ligand
  6. CD30 Ligand/TNFSF8 蛋白, Human (HEK293, mFc)

CD30 Ligand/TNFSF8 蛋白, Human (HEK293, mFc)

目录号: HY-P78405
产品使用指南

CD30L 蛋白是 B 细胞表面抗原,是 CD30 (TNFRSF8) 配体,对 CD40 介导的免疫球蛋白类转换起抑制作用。CD30 Ligand 也是霍奇金淋巴瘤和相关血液恶性肿瘤的标记物,参与 T 细胞依赖性免疫系统的激活和功能。CD30L 蛋白在 T 和 B 淋巴细胞、巨噬细胞、各种正常造血细胞和衍生肿瘤中表达。CD30L 蛋白调控着正常和恶性淋巴样细胞的死亡、分化和细胞分裂。而来源人的 CD30L 蛋白是一种 II 型跨膜糖蛋白,属于肿瘤坏死因子 (TNF) 家族。重组人 CD30 Ligand/TNFSF8 蛋白 (HEK293, mFc) 全长 172 个氨基酸 (Q63-D234),在 HEK293 细胞中表达,带有 N 端 mFc 标签。

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100 μg   询价  

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描述

CD30 Ligand is a B cell surface antigen and a ligand for CD30 (TNFRSF8), playing an inhibitory role in CD40-mediated immunoglobulin class switching[1]. CD30 Ligand is also a a marker for Hodgkin lymphoma and related hematologic malignancies, involves in activation and functioning of the T cell-dependent immune system[2]. CD30 ligand is expressed by T and B lymphocytes, macrophages, and a variety of normal haematopoietic cells and derived tumours. CD30 ligand exerts pleiotropic effects on normal and malignant lymphoid cells, including death, differentiation, or cell division[3]. As for CD30 ligand in human is a type II membrane-associated glycoprotein belonging to the tumor necrosis factor (TNF) family. CD30 Ligand/TNFSF8 Protein, Human (HEK293, mFc) is 172 amino acids in length (Q63-D234) and is expressed in the HEK293 cells with a N-terminal mFc-tag.

研究背景

CD30 Ligand (CD30L) is a B cell surface antigen and a ligand for CD30 (TNFRSF8), playing an inhibitory role in CD40-mediated immunoglobulin class switching[1].
CD30L is a type II membrane-associated glycoprotein belonging to the tumor necrosis factor (TNF) family, structurally related to tumour necrosis superfamily members TNF alpha, TNF beta, and CD40[1][3].
CD30L enhances cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines to play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. CD30L also enhances release of cytokine IL-6, TNF, LT-a[2].
CD30L exerts pleiotropic effects on normal and malignant lymphoid cells, including death, differentiation, or cell division regulation[3].
CD30L is mainly expressed on activated T cells, B cells, macrophages and DCs, while CD30/CD30L mainly expressed on the surface of activated CD4+ T cells in the lamina propria (LP), especially at the early stage of Th17 cell differentiation. CD30L deficiency could inhibit Th17 cell differentiation and production of IL-17A in the intestinal mucosa[4].
CD30L acts as a pro-inflammatory cytokines, is involved in the adaptive immune response in ulcerative colitis (UC), the level of which shows positive correlation with the severity of UC[5].

生物活性

Immobilized Human CD30 hFc at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Human CD30 Ligand mFc with the EC50 of 0.12 μg/mL determined by ELISA.

种属

Human

表达系统

HEK293

标签

N-mFc

蛋白编号

P32971 (Q63-D234)

中文名
重组人 CD30 Ligand/TNFSF8 蛋白 (HEK293, mFc)
同用名
CD153; CD30 Ligand; CD30L; CD30-L; CD30LGMGC138144; TNFSF8; CD30LG
分子量

62-68 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

Lyophilized powder.

组分

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD30 Ligand/TNFSF8 Protein, Human (HEK293, mFc)
目录号:
HY-P78405
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