CD367/CLEC4A 蛋白, Rat (HEK293, His)

The CD367/CLEC4A protein is a C-type lectin receptor that prefers to bind mannose and fucose and interacts weakly with GlcNAc in a Ca(2+)-dependent manner. It is critical for immune regulation, undergoing clathrin-dependent endocytosis upon antigen triggering, leading to antigen presentation and cross-priming of CD8+ T cells. CD367/CLEC4A Protein, Rat (HEK293, His) is the recombinant rat-derived CD367/CLEC4A protein, expressed by HEK293 , with N-His labeled tag. The total length of CD367/CLEC4A Protein, Rat (HEK293, His) is 166 a.a., with molecular weight of 25-33 kDa.

CD367/CLEC4A 蛋白作为 C 型凝集素受体发挥作用，优先结合碳水化合物甘露糖和岩藻糖，并以 Ca(2+) 依赖性方式与 N-乙酰氨基葡萄糖 (GlcNAc) 相互作用较弱。该受体在调节免疫反应中起着至关重要的作用，并且在抗原触发后，通过网格蛋白依赖性内吞作用进行内化。因此，它将其抗原货物传递到抗原呈递途径中，导致 CD8(+) T 细胞的交叉引发。值得注意的是，TLR7 和 TLR8 激动剂增强了这种交叉呈递和交叉引发，导致 CD8(+) T 细胞扩增增加，IFNG 和 TNF 产量增加，同时降低 IL4、IL5 和 IL13 水平。在浆细胞样树突状细胞中，CD367/CLEC4A 抑制 TLR9 介导的 IFNA 和 TNF 产生。此外，它可能通过其 ITIM 基序（基于免疫受体酪氨酸的抑制基序）参与抑制 B 细胞受体介导的钙动员和蛋白质酪氨酸磷酸化。在微生物感染的情况下，CD367/CLEC4A 参与 HIV-1 病毒和树突状细胞之间的相互作用，通过涉及磷酸酶 PTPN6 和 PTPN11、SYK、Src 的 ITIM 基序相关信号转导途径增强 HIV-1 结合和病毒感染激酶和 MAP 激酶。

Rat

HEK293

N-6*His

Q5YIS1 (Q70-S235)

297584  [NCBI]

QKYSQLLEEKKALTDKTLNDLNCTKNVSLTEDKACSCCLKDWKSFGSYCYFTSTDSKATWDESKEKCSRMGAHLLVIHSQDEQDFINTILNIGTDYFIGLSDHSENQWQWIDQTPYNESVTFWHKGEPNNKEEKCVVINHRDKWGWNDIPCHDRHKSVCQVKKIHS

Approximately 25-33 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.