CD4 蛋白, Rat (HEK293, His)

CD4 Protein is expressed on the surface of helper T cells. It plays a key role in immune responses by binding to major histocompatibility complex class II molecules on antigen-presenting cells, facilitating T cell activation and cytokine production. CD4 Protein is also involved in immune regulation and tolerance. Understanding its functions can aid in developing therapies for autoimmune diseases and immunodeficiencies. CD4 Protein, Rat (HEK293, His) is the recombinant rat-derived CD4 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD4 Protein, Rat (HEK293, His) is 367 a.a., with molecular weight of ~42.5-52 kDa.

CD4 是一种完整的膜糖蛋白，在协调针对外部和内部威胁的免疫反应中发挥着至关重要的作用。CD4 主要充当 T 细胞中 MHC II 类分子：肽复合物的辅助受体，有助于识别源自细胞外蛋白的抗原。CD4 同时与抗原呈递细胞 (APC) 上的 T 细胞受体 (TCR) 和 MHC II 类相互作用，将 Src 激酶 LCK 募集至 TCR-CD3 复合物，启动细胞内信号传导途径，最终导致淋巴因子产生、细胞运动、 T 辅助细胞的粘附和激活。除了 T 细胞之外，CD4 通过 TCR/LCK 独立途径促进巨噬细胞或 NK 细胞的分化、激活、细胞因子表达和细胞迁移。它还在 T 辅助细胞的胸腺发育中发挥作用，并触发单核细胞分化为功能性成熟巨噬细胞。该蛋白在细胞表面形成二硫键连接的同二聚体，并与 LCK、PTK2/FAK1、P4HB/PDI 和 IL16 相互作用。此外，CD4 与 MHCII α 和 β 链结合，增强 TCR 对肽-MHCII 的亲和力，并通过 CD4 寡聚化促进 T 细胞和 APC 之间的粘附。

Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.2094 μg/mL, corresponding to a specific activity is 4.776×10³ units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.2094 μg/mL, corresponding to a specific activity is 4.776×10³ units/mg.

Rat

HEK293

C-His

P05540 (K28-T394)

24932  [NCBI]

KTVVLGKEGGSAELPCESTSRRSASFAWKSSDQKTILGYKNKLLIKGSLELYSRFDSRKNAWERGSFPLIINKLRMEDSQTYVCELENKKEEVELWVFRVTFNPGTRLLQGQSLTLILDSNPKVSDPPIECKHKSSNIVKDSKAFSTHSLRIQDSGIWNCTVTLNQKKHSFDMKLSVLGFASTSITAYKSEGESAEFSFPLNLGEESLQGELRWKAEKAPSSQSWITFSLKNQKVSVQKSTSNPKFQLSETLPLTLQIPQVSLQFAGSGNLTLTLDRGILYQEVNLVVMKVTQPDSNTLTCEVMGPTSPKMRLILKQENQEARVSRQEKVIQVQAPEAGVWQCLLSEGEEVKMDSKIQVLSKGLNQT

Approximately 42.5-52 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.