1. 重组蛋白
  2. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens
  3. TNF Superfamily Stimulatory Immune Checkpoint Molecules T Cell CD Proteins Macrophage CD Proteins
  4. TNF Superfamily Ligands CD40 Ligand/CD154
  5. CD40 Ligand
  6. CD40L/CD154/TRAP Trimer 蛋白, Mouse (HEK293, Fc)

CD40L/CD154/TRAP Trimer 蛋白, Mouse (HEK293, Fc)

目录号: HY-P78271
COA 产品使用指南

CD40L (CD154; TRAP) 是 CD40 (TNFRSF5) 的配体,在 T 细胞中产生共刺激信号而发挥上调 IL-4 合成的作用。CD40L 在活化的 CD4+ T 淋巴细胞上特异性表达,参与 NF-κB/MAPK 信号通路。CD40L 还参与 B 细胞的分化、成熟和凋亡。小鼠中 CD40L 蛋白可裂解为 2 种亚型,包括膜状肽 (1-260 a.a.) 和可溶性肽 (112-260 a.a.) (作为整合素的配体)。重组小鼠 CD40L/CD154/TRAP 蛋白三聚体 (HEK293, Fc) 全长 149 个氨基酸 (M112-L260),由 HEK293 细胞表达,带有 N 端 hFc 标签。

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20 μg ¥1150 In-stock
50 μg ¥2500 In-stock
100 μg   询价  

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描述

CD40L (CD154; TRAP) is a ligand to CD40/TNFRSF5, acts function by generating a costimulatory signal that up-regulates IL-4 synthesis[1]. CD40L is specifically expressed on activated CD4+ T-lymphocytes and involves in activation of NF-κB/MAPK pathway[2][3]. CD40L also involves in B cell differentiation, maturation, and apoptosis[4]. CD40L in mouse, cleaved into 2 chains of membrane form (1-260 a.a.) and soluble form (112-260 a.a.) which serves as a ligand for integrins (ITGA5:ITGB1 and ITGAV:ITGB3). CD40L/CD154/TRAP Trimer Protein, Mouse (HEK293, Fc) has a total length of 149 amino acids (M112-L260), is expressed in HEK392 cells with N-terminal hFc-tag.

研究背景

CD40 配体(CD40L;CD154;TRAP)属于肿瘤坏死因子 (TNF) 家族,是 CD40/TNFRSF5 的配体,特异性表达于活化的 CD4+ T 淋巴细胞[1]
CD40L 是 B 细胞上的一种 II 型跨膜蛋白,可触发 B 细胞分化、成熟和凋亡的重要信号[4]
CD40L 通过与 T 细胞交联来发挥作用产生共刺激信号,从而与 TCR/CD3 连接和 CD28 共刺激一起增强 IL4 和 IL10 的产生,并促进干扰素-γ 和 TNF-α[1] 的产生[4]
CD40L 与抗原呈递细胞 (APC) 上的 CD40 结合,通过刺激 I-κB 激酶 (IKK) 激活 TNFR 相关因子 2 和 IKK2 依赖性通路,增加 NF-κB DNA 结合和 p65 核转位。I-κB 激酶的激活导致 c-Jun N 末端激酶强烈激活以及 GST-I-κB 和 GST-p65 磷酸化[2]
CD40L 参与 MAPK强烈抑制 Bcl-6 的途径,诱导 Erk1/2、p38 和 Jnk1/2 磷酸化,并激活 NF-κB[3] 介导的 IRF4。
CD40L 还结合并发出信号通过几个整合素,包括 αvβ3α5β1,它们与 CD40L 的三聚体界面结合。CD40L 在免疫反应中发挥重要作用,是炎症的主要靶点[5]
CD40L 广泛存在于不同动物中,而小鼠中的序列与大鼠高度相似(93.85 %),但与人类和恒河猴有很大不同,相似度分别为 77.69% 和 77.31%。小鼠中的CD40L被切割成膜形式(1-260个氨基酸)和可溶性形式(112-260个氨基酸)的2条链,而人中的可溶性形式通过蛋白水解加工源自膜形式。血小板中可溶性 CD40L 的释放部分受到 GP IIb/IIIa、肌动蛋白聚合和基质金属蛋白酶 (MMP) 抑制剂敏感途径[6]的调节。

体外研究
(In Vitro)

CD40 Ligand lacking in mice (CD40L-deficient) results CLP-induced edema and myeloperoxidase activity in the lung as well as neutrophil infiltration in the broncoalveolar space markedly reduced[7].

体内研究
(In Vivo)

CD40 Ligand (100 ng/mL; 10 min) doesn’t stimulate Mac-1 expression in murine blood neutrophils indicating a MIP-2-dependent manner[7].
CD40 Ligand (100 ng/mL; 10 min) regulates plasma levels of MIP-2 in plasma of cecal ligation and puncture (CLP) mice[7].

生物活性

Immobilized Mouse CD40, His Tag at 1 μg/ml (100 μL/Well) on the plate. Dose response curve for Mouse CD40 Ligand (Trimer), hFc Tag with the EC50 of 0.10 μg/ml determined by ELISA.

种属

Mouse

表达系统

HEK293

标签

N-hFc

蛋白编号

P27548 (M112-L260)

基因 ID

21947  [NCBI]

蛋白结构
N-term
hFc
CD40L (M112-L260)
Accession # P27548
C-term
中文名
重组小鼠 CD40L/CD154/TRAP 蛋白三聚体 (HEK293, Fc)
同用名
CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
氨基酸序列

MQRGDEDPQIAAHVVSEANSNAASVLQWAKKGYYTMKSNLVMLENGKQLTVKREGLYYVYTQVTFCSNREPSSQRPFIVGLWLKPSSGSERILLKAANTHSSSQLCEQQSVHLGGVFELQAGASVFVNVTEASQVIHRVGFSSFGLLKL

分子量

68-80 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD40L/CD154/TRAP Trimer Protein, Mouse (HEK293, Fc)
目录号:
HY-P78271
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