CD44 蛋白, Human (HEK293, His)

CD44 is a cell surface receptor that plays a key role in calcium mobilization and actin-mediated cytoskeletal reorganization, cell migration, and adhesion. CD44 Protein, Human (HEK293, His) is the recombinant human-derived CD44 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CD44 Protein, Human (HEK293, His) is 200 a.a., with molecular weight of 38-50 kDa.

CD44 是一种细胞表面受体，在细胞间相互作用、粘附和迁移中发挥着关键作用，使细胞能够感知并响应组织微环境的变化。它涉及多种细胞功能，包括 T 淋巴细胞的激活、再循环和归巢、造血、炎症和对细菌感染的反应。CD44 通过其胞外域结合各种细胞外基质成分，如乙酰透明质酸/HA、胶原蛋白、生长因子、细胞因子或蛋白酶，作为信号转导的平台。这涉及通过其细胞质结构域组装蛋白质复合物，其中包含受体激酶和膜蛋白酶。PKN2、RAC1、RHOA、Rho 激酶和磷脂酶 C 等效应器与 CD44 协调信号传导通路，导致钙动员和肌动蛋白介导的细胞骨架重组，这对细胞迁移和粘附至关重要。CD44 与一系列分子相互作用，包括 PKN2、TIAM1、TIAM2、透明质酸、胶原蛋白、层粘连蛋白、纤连蛋白、UNC119、PDPN、RDX、EZR、MSN、EGFR 和 CD74，形成对其多种细胞功能至关重要的复杂网络。

Measured in a cell proliferation assay using A549 cells. The ED₅₀ this effect is 1.543 ng/ml, corresponding to a specific activity is 6.481×10⁵ units/mg.

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  Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 1.543 ng/mL, corresponding to a specific activity is 6.481×10⁵ units/mg.

Human

HEK293

C-6*His

P16070 (Q21-P220)

960  [NCBI]

QIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIP

38-50 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.