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  4. CD73
  5. CD73/5'-Nucleotidase 蛋白, Human (T376A, HEK293, Fc)

CD73/5'-Nucleotidase 蛋白, Human (T376A, HEK293, Fc)

目录号: HY-P74272
产品使用指南

CD73/5'-核苷酸酶蛋白水解核苷酸单磷酸,有利于 AMP 作为主要底物,并显示出对核糖核苷酸单磷酸的偏好。它释放无机磷酸盐和相应的核苷。CD73 还作用于其他底物,包括 IMP、UMP、GMP、CMP、dAMP、dCMP、dTMP、NAD 和 NMN。CD73/5'-Nucleotidase 蛋白, Human (T376A, HEK293, Fc) 是重组的 CD73/5'-核苷酸酶蛋白,由 HEK293 表达,带有 C-hFc 标签。CD73/5'-Nucleotidase 蛋白, Human (T376A, HEK293, Fc) 全长 547 个氨基酸,分子量约为 ~84.5 kDa。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

CD73/5'-Nucleotidase Protein hydrolyzes nucleotide monophosphates, favoring AMP as the primary substrate and showing a preference for ribonucleotide monophosphates. It releases inorganic phosphate and the corresponding nucleoside. CD73 also acts on other substrates, including IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD, and NMN. CD73/5'-Nucleotidase Protein, Human (T376A, HEK293, Fc) is the recombinant human-derived CD73/5'-Nucleotidase protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD73/5'-Nucleotidase Protein, Human (T376A, HEK293, Fc) is 547 a.a., with molecular weight of ~84.5 kDa.

研究背景

CD73/5'-核苷酸酶蛋白通过催化单磷酸核苷酸的水解、释放无机磷酸盐和相应的核苷,在细胞过程中发挥着至关重要的作用。值得注意的是,AMP 是该酶的首选底物,强调了它在腺苷酸核苷酸转化中的重要性。CD73/5'-核苷酸酶表现出对核糖核苷酸单磷酸的偏好超过其脱氧核糖对应物,显示其在底物识别中的选择性。除AMP外,其他底物还包括IMP、UMP、GMP、CMP、dAMP、dCMP、dTMP、NAD和NMN,进一步说明CD73/5'-核苷酸酶在核苷酸代谢中的多功能性及其调节核苷酸平衡的作用细胞内的池。

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

P21589 (M1-K547, T376A)

基因 ID
蛋白结构
N-term
CD73 (M1-K547, T376A)
Accession # P21589
hFc
C-term
同用名
5-NT; 5'-Nucleotidase; CD73; E5NT; NT5E; NTE
分子量

Approximately 84.5 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD73/5'-Nucleotidase Protein, Human (T376A, HEK293, Fc)
目录号:
HY-P74272
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