CLIC4/Chloride intracellular channel 4 蛋白, Human (His)

CLIC4/Chloride The intracellular channel 4 protein inserts into the membrane to form a pH-dependent, poorly selective ion channel that transports chloride ions. It enhances cell surface expression of HRH3 and maintains membrane polarity during angiogenesis, mitosis, cytokinesis, endothelial cell proliferation, and morphogenesis. CLIC4/Chloride intracellular channel 4 Protein, Human (His) is the recombinant human-derived CLIC4/Chloride intracellular channel 4 protein, expressed by E. coli , with N-6*His labeled tag. The total length of CLIC4/Chloride intracellular channel 4 Protein, Human (His) is 253 a.a., with molecular weight of ~32.0 kDa.

CLIC4，被称为氯离子细胞内通道4，能够插入细胞膜，形成选择性差的离子通道，可能促进氯离子转运。其通道活性依赖于 pH 值，并且其膜插入似乎受氧化还原调节，特别是在氧化条件下发生。除了离子转运之外，CLIC4 还发挥多种作用，包括促进 HRH3 的细胞表面表达并参与各种细胞功能，例如血管生成、有丝分裂和胞质分裂期间顶基底外侧膜极性的维持以及内皮细胞增殖和形态发生的调节。它是涉及肌动蛋白、埃兹蛋白、α-肌动蛋白、凝溶胶蛋白、IQGAP1 和 CLIC5A 等细胞骨架蛋白的多聚体复合物的一部分。CLIC4 在包含肌动蛋白、微管蛋白和 14-3-3 亚型的复合物中直接与脑动力 I 相互作用，并且还与 HRH3 和 AKAP9 相互作用。

Human

E. coli

N-6*His

Q9Y696 (M1-K253)

25932  [NCBI]

MALSMPLNGLKEEDKEPLIELFVKAGSDGESIGNCPFSQRLFMILWLKGVVFSVTTVDLKRKPADLQNLAPGTHPPFITFNSEVKTDVNKIEEFLEEVLCPPKYLKLSPKHPESNTAGMDIFAKFSAYIKNSRPEANEALERGLLKTLQKLDEYLNSPLPDEIDENSMEDIKFSTRKFLDGNEMTLADCNLLPKLHIVKVVAKKYRNFDIPKEMTGIWRYLTNAYSRDEFTNTCPSDKEVEIAYSDVAKRLTK

Approximately 32.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.