1. 重组蛋白
  2. Receptor Proteins
  3. G-Protein-Coupled Receptors (GPCRs)
  4. Muscarinic Acetylcholine Receptor
  5. Cholinergic Receptor Muscarinic 1 (CHRM1)
  6. CHRM1 蛋白, Rat (His)

CHRM1 蛋白, Rat (His)

目录号: HY-P72138
产品使用指南

CHRM1 是毒蕈碱乙酰胆碱受体,介导细胞反应,包括腺苷酸环化酶抑制、磷酸肌醇分解和通过 G 蛋白作用调节钾通道。它与 GPRASP2 和 TMEM147 结合,形成由受体调节的信号通路和细胞过程的复杂网络。CHRM1 蛋白, Rat (His) 是重组的 CHRM1 蛋白,由 E. coli 表达,带有 N-6*His 标签。CHRM1 蛋白, Rat (His) 全长 157 个氨基酸,分子量约为 ~21.1 kDa。

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     可免费申领三个不同产品的试用装。

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描述

CHRM1, the muscarinic acetylcholine receptor, mediates cellular responses, including adenylate cyclase inhibition, phosphoinositide breakdown, and potassium channel modulation through G protein actions. It engages with GPRASP2 and TMEM147, contributing to a complex network of signaling pathways and cellular processes regulated by the receptor. CHRM1 Protein, Rat (His) is the recombinant rat-derived CHRM1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of CHRM1 Protein, Rat (His) is 157 a.a., with molecular weight of ~21.1 kDa.

研究背景

以 CHRM1 为代表的毒蕈碱乙酰胆碱受体负责介导多种细胞反应,例如抑制腺苷酸环化酶、磷酸肌醇分解以及通过 G 蛋白介导的作用调节钾通道。其主要转换效应涉及 Pi 周转。此外,CHRM1 与 GPRASP2 和 TMEM147 相互作用,形成由该受体调节的信号通路和细胞过程的复杂网络。

种属

Rat

表达系统

E. coli

标签

N-6*His

蛋白编号

P08482 (R210-T366)

基因 ID

25229  [NCBI]

蛋白结构
N-term
6*His
CHRM1 (R210-T366)
Accession # P08482
C-term
同用名
Chrm1; Chrm-1Muscarinic acetylcholine receptor M1
氨基酸序列

RIYRETENRARELAALQGSETPGKGGGSSSSSERSQPGAEGSPESPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDSEAQAPTKQPPKSSPNTVKRPTKKGRDRGGKGQKPRGKEQLAKRKTFSLVKEKKAART

分子量

Approximately 21.1 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CHRM1 Protein, Rat (His)
目录号:
HY-P72138
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