1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. CK1d 蛋白, Human (Sf9, GST)

CK1d 蛋白, Human (Sf9, GST)

目录号: HY-P701658
产品使用指南

CK1d 蛋白是一种重要的丝氨酸/苏氨酸蛋白激酶,可复杂地控制 Wnt 信号传导、DNA 修复和昼夜节律等细胞过程。其多功能性在磷酸化多种蛋白质方面表现明显,包括 connexin-43/GJA1、MAP1A、p53/TP53 和 YAP1。CK1d 蛋白, Human (Sf9, GST) 是重组的 CK1d 蛋白,由 Sf9 insect cells 表达,带有 N-GST 标签。CK1d 蛋白, Human (Sf9, GST) 全长 414 个氨基酸。

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1.  客户无需承担相应的运输费用。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

The CK1d protein is an important serine/threonine protein kinase that complexly controls cellular processes such as Wnt signaling, DNA repair, and circadian rhythms. Its versatility is evident in phosphorylating a variety of proteins, including connexin-43/GJA1, MAP1A, p53/TP53, and YAP1. CK1d Protein, Human (Sf9, GST) is the recombinant human-derived CK1d protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of CK1d Protein, Human (Sf9, GST) is 414 a.a., .

研究背景

CK1d 蛋白是一种必需的丝氨酸/苏氨酸蛋白激酶,复杂地调节细胞生长和生存过程,包括 Wnt 信号传导、DNA 修复和昼夜节律。CK1d 具有多功能性,可磷酸化多种蛋白质,包括 connexin-43/GJA1、MAP1A、SNAPIN、MAPT/TAU、TOP2A、DCK、HIF1A、EIF6、p53/TP53、DVL2、DVL3、ESR1、AIB1/NCOA3、DNMT1、 PKD2、YAP1、PER1 和 PER2。它在生物钟中发挥着核心作用,通过调节 PER1 和 PER2 磷酸化来确定周期长度。此外,CK1d 影响 WNT3A 信号通路、神经元生长、YAP1 泛素化、DNMT1 的 DNA 结合活性以及许多其他细胞过程。这种多方面的激酶在协调不同的细胞功能和信号通路中发挥着关键作用。

种属

Human

表达系统

Sf9 insect cells

标签

N-GST

蛋白编号

P48730 (E2-R415)

基因 ID

1453

蛋白结构
N-term
GST
CK1d (E2-R415)
Accession # P48730
C-term
同用名
CSNK1D; Casein kinase I isoform delta; CKI-delta; CKId; Tau-protein kinase CSNK1D
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CK1d Protein, Human (Sf9, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CK1d Protein, Human (Sf9, GST)
目录号:
HY-P701658
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