CNR1 Protein-VLP, Human (HEK293, His)

CNR1 Protein-VLP, Human (HEK293, His) is recommended for animal immunization, ELISA. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P701236. Tags can only be detected under denaturing conditions.

CNR1 蛋白-VLP 充当内源性大麻素 (eCB) 的 G 蛋白偶联受体，包括 N-花生四烯酰乙醇酰胺（anandamide 或 AEA）和 2-花生四烯酰甘油 (2-AG)，以及植物大麻素，如 delta(9)-四氢大麻酚（四氢大麻酚）。它介导一系列大麻素引起的对食物摄入、记忆、胃肠道运动、僵直、行走活动、焦虑和慢性疼痛的影响，通常涉及环磷酸腺苷的减少。在下丘脑中，它对线粒体呼吸表现出双重作用，通过 G 蛋白 α-i 蛋白激活和随后抑制线粒体可溶性腺苷酸环化酶，在低剂量时增加呼吸，在高剂量时减少呼吸。此外，CNR1 抑制瘦素诱导的活性氧 (ROS) 形成，介导大麻素诱导的 SREBF1 和 FASN 基因表达增加，并驱动下丘脑 POMC 神经元释放促进食欲的 β-内啡肽。在海马体中，它响应大麻素调节细胞呼吸和能量产生，并参与大麻素依赖性过程，如去极化诱导的抑制抑制 (DSI)。CNR1 还抑制颈上神经节和脑血管平滑肌细胞中的电压门控 Ca(2+) 通道，导致血管舒张和血管张力降低。它诱导脂肪细胞中瘦素的产生，减少肾脏中 LRP2 介导的瘦素清除率，并调节脂肪组织和肝脏中的脂质代谢。此外，CNR1 外周调节能量代谢，并且在高碳水化合物饮食引起的肥胖中，可能影响横纹肌中线粒体二氢硫辛酰脱氢酶/DLD 表达和选定的葡萄糖/丙酮酸代谢酶。作为对大麻素 anandamide 的反应，CNR1 在巨噬细胞中引发促炎反应，可能导致 2 型糖尿病的进展和胰腺 β 细胞的损失。值得注意的是，CNR1 结合 2-花生四烯酰甘油 (2-AG) 和花生四烯酸乙醇胺。

Measured by its binding ability in a functional ELISA. Immobilized Human CNR1-VLPs at 1 μg/mL can bind Anti-CNR1 recombinant antibody , the EC₅₀ is <85 ng/mL.

Human

HEK293

C-10*His

P21554 (M1-L472)

1268  [NCBI]

Full Length

MKSILDGLADTTFRTITTDLLYVGSNDIQYEDIKGDMASKLGYFPQKFPLTSFRGSPFQEKMTAGDNPQLVPADQVNITEFYNKSLSSFKENEENIQCGENFMDIECFMVLNPSQQLAIAVLSLTLGTFTVLENLLVLCVILHSRSLRCRPSYHFIGSLAVADLLGSVIFVYSFIDFHVFHRKDSRNVFLFKLGGVTASFTASVGSLFLTAIDRYISIHRPLAYKRIVTRPKAVVAFCLMWTIAIVIAVLPLLGWNCEKLQSVCSDIFPHIDETYLMFWIGVTSVLLLFIVYAYMYILWKAHSHAVRMIQRGTQKSIIIHTSEDGKVQVTRPDQARMDIRLAKTLVLILVVLIICWGPLLAIMVYDVFGKMNKLIKTVFAFCSMLCLLNSTVNPIIYALRSKDLRHAFRSMFPSCEGTAQPLDNSMGDSDCLHKHANNAASVHRAAESCIKSTVKIAKVTMSVSTDTSAEAL

54.6 kDa

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.