1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. Creatine kinase B-type/CKB 蛋白, Human (His)

Creatine kinase B-type/CKB 蛋白, Human (His)

目录号: HY-P7897
COA 产品使用指南

Creatine kinaseB-type/CKB 蛋白, Human (His) 是纯化的棕色脂肪细胞中含量最丰富的肌酸激酶同工酶。CKB 可逆地催化 ATP 和各种磷原之间的磷酸盐转移。

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规格 价格 是否有货 数量
10 μg ¥551 In-stock
50 μg ¥1654 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

Creatine kinaseB-type/CKB Protein, Human (His) is the most abundant creatine kinase isoenzyme in purified brown adipocytes. CKB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens.

研究背景

肌酸激酶 B (CKB) 对于无效肌酸循环产生的产热是不可或缺的,在此期间,它使用内部线粒体靶向序列转运至线粒体。CKB 在小鼠和人类脂肪细胞中均受到产热刺激的强烈诱导。小鼠脂肪细胞选择性失活 Ckb 会降低产热能力,增加肥胖倾向,并破坏葡萄糖稳态。因此,CKB 是无效肌酸循环的关键效应器[1]

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P12277 (M1-K381)

基因 ID
蛋白结构
N-term
6*His
CKB (M1-K381)
Accession # P12277
C-term
中文名
重组人B型肌酸激酶(CKB)
同用名
rHuCreatine kinase B-type/CKB , His; B-CK; CKB; Creatine Kinase BB
氨基酸序列

MPFSNSHNALKLRFPAEDEFPDLSAHNNHMAKVLTPELYAELRAKSTPSGFTLDDVIQTGVDNPGHPYIMTVGCVAGDEESYEVFKDLFDPIIEDRHGGYKPSDEHKTDLNPDNLQGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCSRGERRAIEKLAVEALSSLDGDLAGRYYALKSMTEAEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKTFLVWVNEEDHLRVISMQKGGNMKEVFTRFCTGLTQIETLFKSKDYEFMWNPHLGYILTCPSNLGTGLRAGVHIKLPNLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVFDVSNADRLGFSEVELVQMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK

分子量

Approximately 50.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 7.5.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献

Creatine kinase B-type/CKB Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Creatine kinase B-type/CKB Protein, Human (His)
目录号:
HY-P7897
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