1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. Creatine kinase M-type/CKM 蛋白, Human (HEK293, His)

Creatine kinase M-type/CKM 蛋白, Human (HEK293, His)

目录号: HY-P7827
COA 产品使用指南

KCRM 是一种细胞质肌酸激酶同工酶,负责参与能量转导和维持能量稳态。KCRM 能可逆地催化 ATP 和各种磷原(例如磷酸肌酸)之间的磷酸盐转移,是心肌梗塞的重要血清标志物。Creatine kinase M-type/CKM 蛋白, Human (HEK293, His) 是重组的 肌酸激酶 M 型/CKM 蛋白,由 HEK293 表达,带有 C-6*His 标签。Creatine kinase M-type/CKM 蛋白, Human (HEK293, His) 全长 381 个氨基酸,分子量约为 ~46.0 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μg ¥1000 In-stock
50 μg ¥3000 In-stock
100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

KCRM is a cytoplasmic creatine kinase isoenzyme responsible for participating in energy transduction and maintaining energy homeostasis. KCRM reversibly catalyzes phosphate transfer between ATP and various phosphogens (e.g., creatine phosphate) and is an important serum marker of myocardial infarction. Creatine kinase M-type/CKM Protein, Human (HEK293, His) is the recombinant human-derived Creatine kinase M-type/CKM protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Creatine kinase M-type/CKM Protein, Human (HEK293, His) is 381 a.a., with molecular weight of ~46.0 kDa.

研究背景

KCRM 是一种细胞质肌酸激酶同工酶,可逆地催化 ATP 和各种磷原(例如磷酸肌酸)之间的磷酸盐转移。KCRM 在能量需求较大且波动的组织(例如骨骼肌、心脏、大脑和精子)中,参与能量转导和能量稳态,是心肌梗塞的重要血清标志物。KCRM 在横纹肌和其他组织中充当同二聚体,并在心脏中充当具有类似脑同工酶的异二聚体。骨骼肌作为重要的分泌器官,在 2 型糖尿病(T2DM)骨骼肌组织中差异分泌裂解的 KCRM 肽。KCRM 发生自然发生的突变可能导致具有活性血清肌酸激酶的个体对 Tenofovir (HY-13910) 暴露前预防 (PrEP) HIV 没有反应。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

AAP35439.1 (M1-K381)

基因 ID
蛋白结构
N-term
CKM (M1-K381)
Accession # AAP35439.1
6*His
C-term
同用名
rHuCreatine kinase M-type/CKMM, His; Creatine kinase M-type; Creatine kinase M chain; M-CK; CKM; CKMM
氨基酸序列

MPFGNTHNKFKLNYKPEEEYPDLSKHNNHMAKVLTLELYKKLRDKETPSGFTVDDVIQTGVDNPGHPFIMTVGCVAGDEESYEVFKELFDPIISDRHGGYKPTDKHKTDLNHENLKGGDDLDPNYVLSSRVRTGRSIKGYTLPPHCSRGERRAVEKLSVEALNSLTGEFKGKYYPLKSMTEKEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKSFLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLQKIEEIFKKAGHPFMWNQHLGYVLTCPSNLGTGLRGGVHVKLAHLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVFDVSNADRLGSSEVEQVQLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK

分子量

Approximately 46.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl,150 mM NaCl, 10% Glycerol, pH7.5.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Creatine kinase M-type/CKM Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Creatine kinase M-type/CKM Protein, Human (HEK293, His)
目录号:
HY-P7827
需求量: