1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. CROT 蛋白, Human (V474L, sf9, His)

CROT 蛋白, Human (V474L, sf9, His)

目录号: HY-P74218
产品使用指南

CROT 蛋白在 β-氧化过程中至关重要,对 C6 至 C10 底物显示出最高的酶活性。作为脂质代谢的关键参与者,CROT 促进 4,8-二甲基壬酰辅酶 A 转化为其肉碱酯,调节脂肪酸氧化以实现细胞能量平衡。了解 CROT 的具体活动揭示了控制脂肪酸分解代谢的机制,对代谢调节和能量产生具有潜在影响。对 CROT 功能的进一步研究为代谢紊乱的治疗干预带来了希望。CROT 蛋白, Human (V474L, sf9, His) 是重组的 CROT 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签和 V474L 突变。CROT 蛋白, Human (V474L, sf9, His) 全长 612 个氨基酸,分子量约为 ~65 kDa。

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描述

CROT Protein is pivotal in beta-oxidation, displaying highest enzymatic activity on C6 to C10 substrates. As a key player in lipid metabolism, CROT facilitates the conversion of 4,8-dimethylnonanoyl-CoA to its carnitine ester, regulating fatty acid oxidation for cellular energy balance. Understanding CROT's specific activities unveils mechanisms governing fatty acid catabolism, with potential implications for metabolic regulation and energy production. Further investigation into CROT's function holds promise for therapeutic interventions in metabolic disorders. CROT Protein, Human (V474L, sf9, His) is the recombinant human-derived CROT protein, expressed by Sf9 insect cells , with C-His labeled tag and V474L mutation. The total length of CROT Protein, Human (V474L, sf9, His) is 612 a.a., with molecular weight of ~65 kDa.

研究背景

CROT 蛋白在脂肪酸的 β 氧化中发挥着关键作用,对链长范围为 C6 至 C10 的底物表现出最高的酶活性。作为脂质代谢的关键参与者,CROT 促进降植烷酸 β-氧化的最终产物(即 4,8-二甲基壬酰辅酶 A)转化为其相应的肉碱酯。这种酶促过程对于调节脂肪酸氧化平衡至关重要,有助于细胞能量稳态。了解 CROT 的具体活动可以深入了解控制脂肪酸分解代谢的复杂机制,对代谢调节和能量产生具有潜在影响。对 CROT 功能的进一步研究有望增进我们对脂质代谢的理解,并可能为代谢紊乱的治疗干预提供途径。

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

Q9UKG9 (M1-L612,V474L)

基因 ID
蛋白结构
N-term
CROT (M1-L612,V474L)
Accession # Q9UKG9
His
C-term
同用名
Peroxisomal carnitine O-octanoyltransferase; CROT; COT
分子量

Approximately 65 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CROT Protein, Human (V474L, sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CROT Protein, Human (V474L, sf9, His)
目录号:
HY-P74218
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