CSEN 蛋白, Human (His)

CSEN is a calcium-dependent transcriptional repressor that binds to the DRE elements of genes such as PDYN and FOS. Its affinity to DNA is affected by calcium. The binding force is reduced in the presence of calcium and enhanced when combined with magnesium. CSEN Protein, Human (His) is the recombinant human-derived CSEN protein, expressed by E. coli , with N-His labeled tag.

CSEN 是一种钙依赖性转录抑制因子，在细胞过程中发挥多方面的作用。它与多种基因的 DRE（下游调节元件）结合，包括 PDYN 和 FOS，在钙结合后表现出与 DNA 的亲和力降低，与镁的亲和力增强。此外，CSEN 与伤害感受有关，表明其可能参与疼痛感知。除了其转录功能外，CSEN 还充当 Kv4/D (Shal) 型电压门控的调节亚基，快速失活 A 型钾通道，例如 KCND2/Kv4.2 和 KCND3/Kv4.3。在这种能力下，它调节细胞膜上的通道表达，影响门控特性、失活动力学和失活恢复率。值得注意的是，这些调节作用以钙依赖性和异构体特异性的方式发挥作用，强调了 CSEN 在钾通道活性中作用的复杂性。

Human

E. coli

N-His

Q9Y2W7-1 (M1-I256)

30818  [NCBI]

MQPAKEVTKASDGSLLGDLGHTPLSKKEGIKWQRPRLSRQALMRCCLVKWILSSTAPQGSDSSDSELELSTVRHQPEGLDQLQAQTKFTKKELQSLYRGFKNECPTGLVDEDTFKLIYAQFFPQGDATTYAHFLFNAFDADGNGAIHFEDFVVGLSILLRGTVHEKLKWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRHTYPILREDAPAEHVERFFEKMDRNQDGVVTIEEFLEACQKDENIMSSMQLFENVI

Approximately 29 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.