1. 重组蛋白
  2. Enzymes & Regulators
  3. Isomerases (EC 5)
  4. Cyclophilin F/PPIF 蛋白, Human (HEK293, His, solution)

Cyclophilin F/PPIF 蛋白, Human (HEK293, His, solution)

目录号: HY-P74211
COA 产品使用指南

Cyclophilin F/PPIF 蛋白是一种肽基脯氨酰顺反异构酶 (PPIase),可催化脯氨酸酰亚胺肽键的异构化,并可能有助于蛋白质折叠。它关键性地调节线粒体通透性转换孔(mPTP),影响细胞凋亡或坏死。Cyclophilin F/PPIF 蛋白, Human (HEK293, His, solution) 是重组的 亲环蛋白 F/PPIF 蛋白,由 HEK293 表达,带有 C-His 标签。Cyclophilin F/PPIF 蛋白, Human (HEK293, His, solution) 全长 178 个氨基酸,分子量约为 ~20.3 kDa。

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描述

Cyclophilin F/PPIF protein is a peptidyl prolyl cis-trans isomerase (PPIase) that catalyzes the isomerization of proline imide peptide bonds and may contribute to protein folding. It critically regulates the mitochondrial permeability transition pore (mPTP), affecting cell apoptosis or necrosis. Cyclophilin F/PPIF Protein, Human (HEK293, His, solution) is the recombinant human-derived Cyclophilin F/PPIF protein, expressed by HEK293 , with C-His labeled tag. The total length of Cyclophilin F/PPIF Protein, Human (HEK293, His, solution) is 178 a.a., with molecular weight of ~20.3 kDa.

研究背景

Cyclophilin F/PPIF 蛋白作为肽基-脯氨酰顺反异构酶 (PPIase) 起作用,在催化寡肽中脯氨酸酰亚胺肽键的顺反异构化中发挥着至关重要的作用,从而可能促进蛋白质折叠。除了参与蛋白质折叠之外,亲环蛋白 F/PPIF 还在线粒体通透性转换孔 (mPTP) 的调节中发挥着关键作用,其中它与 mPTP 的关联被认为掩盖了抑制无机磷酸盐 (Pi) 的结合位点,最终促进mPTP开放概率并导致细胞凋亡或坏死;然而,在此过程中是否需要 PPIase 活性仍存在争议。此外,亲环蛋白 F/PPIF 与线粒体 p53/TP53 合作,有助于激活氧化应激诱导的坏死。它进一步参与调节线粒体膜 F(1)F(0) ATP 合酶活性和调节线粒体基质腺嘌呤核苷酸水平。Cyclophilin F/PPIF 具有独立于 mPTP 的抗凋亡活性,与 BCL2 协同作用,可有效抑制细胞色素 c 依赖性细胞凋亡,突出其在线粒体功能和细胞生存机制中的多方面作用。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P30405 (C30-S207)

基因 ID
蛋白结构
N-term
PPIF (C30-S207)
Accession # P30405
His
C-term
同用名
Peptidyl-prolyl cis-trans isomerase; PPIase; PPIF
分子量

Approximately 20.3 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Cyclophilin F/PPIF Protein, Human (HEK293, His, solution) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Cyclophilin F/PPIF Protein, Human (HEK293, His, solution)
目录号:
HY-P74211
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